Gulati S M, Saha K, Kant L, Thusoo T K, Prakash A
Angiology. 1984 May;35(5):276-81. doi: 10.1177/000331978403500503.
Circulatory immune complexes were estimated in the sera of 25 patients and 20 age and sex matched controls by precipitation with 8% polyethylene glycol (PEG). Characterization of the immune complexes, thus obtained, was performed by the double immuno-diffusion method. The mean protein content of the PEG precipitates was significantly higher in the patients of thromboangiitis obliterans than in the controls. The immunoglobulins in the immune complexes were predominantly of mixed type in contrast to mainly single class immunoglobulins in the controls. C3 complement distribution in immune complexes of patients and control sera was 64% and 45% respectively. The immune complexes in patients were found to be biologically active. The mechanism of formation of circulatory immune complexes and their role in the pathogenesis of arteritis in relation to smoking of tobacco has been postulated.
采用8%聚乙二醇（PEG）沉淀法对25例血栓闭塞性脉管炎患者及20例年龄和性别匹配的对照者血清中的循环免疫复合物进行了测定。采用双向免疫扩散法对由此获得的免疫复合物进行了鉴定。血栓闭塞性脉管炎患者的PEG沉淀物平均蛋白含量显著高于对照组。免疫复合物中的免疫球蛋白以混合型为主，而对照组主要为单类免疫球蛋白。患者和对照血清免疫复合物中的C3补体分布分别为64%和45%。发现患者的免疫复合物具有生物活性。推测了循环免疫复合物的形成机制及其在与吸烟相关的动脉炎发病机制中的作用。
