一种用于研究霍乱弧菌细胞表面抗原的酶联免疫吸附测定法的开发。
Development of an enzyme-linked immunosorbent assay for studying Vibrio cholerae cell surface antigens.
作者信息
Cryz S J, Fürer E, Germanier R
出版信息
J Clin Microbiol. 1982 Jul;16(1):41-5. doi: 10.1128/jcm.16.1.41-45.1982.
Abstract
An enzyme-linked immunosorbent assay for the measurement of antibodies directed against cell surface antigens of Vibrio cholerae (CSA ELISA) was developed. NaN3-killed whole cells of V. cholerae, adsorbed to polystyrene tubes, were used as immobilized antigens. The assay was capable of detecting antibodies directed against lipopolysaccharide and non-lipopolysaccharide surface antigens. In addition, the CSA ELISA was capable of detecting non-vibriocidal antibody. An antiserum raised in rabbits by immunization with live V. cholerae 1418 (Ogawa, El Tor) was capable of reacting with various heterologous strains of V. cholerae used as immobilized antigens. Therefore, common antigens shared by V. cholerae strains could be detected by using the CSA ELISA.
摘要
开发了一种用于测量针对霍乱弧菌细胞表面抗原的抗体的酶联免疫吸附测定法（CSA ELISA）。吸附在聚苯乙烯管上的经叠氮化钠灭活的霍乱弧菌全细胞用作固定抗原。该测定法能够检测针对脂多糖和非脂多糖表面抗原的抗体。此外，CSA ELISA能够检测非杀弧菌抗体。通过用活的霍乱弧菌1418（小川型，埃尔托生物型）免疫家兔产生的抗血清能够与用作固定抗原的各种霍乱弧菌异源菌株发生反应。因此，使用CSA ELISA可以检测霍乱弧菌菌株共有的共同抗原。
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