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J Clin Microbiol. 1982 Jul;16(1):41-5. doi: 10.1128/jcm.16.1.41-45.1982.
2
Enzyme-linked immunoassays for antibodies against Vibrio cholerae.针对霍乱弧菌抗体的酶联免疫测定法。
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4
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3
Effect of chemical and heat inactivation on the antigenicity and immunogenicity of Vibrio cholerae.化学和热灭活对霍乱弧菌抗原性和免疫原性的影响。
Infect Immun. 1982 Oct;38(1):21-6. doi: 10.1128/iai.38.1.21-26.1982.
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Infect Immun. 1988 Jan;56(1):161-7. doi: 10.1128/iai.56.1.161-167.1988.
7
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Infect Immun. 1992 Nov;60(11):4848-55. doi: 10.1128/iai.60.11.4848-4855.1992.

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Antitoxic immunity in experimental cholera: observations with purified antigens and the ligated ileal loop model.实验性霍乱的抗毒免疫：用纯化抗原和结扎回肠袢模型的观察。
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Identification and preliminary characterization of Vibrio cholerae outer membrane proteins.霍乱弧菌外膜蛋白的鉴定与初步表征
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Composition and immunochemical properties of outer membrane proteins of Vibrio cholerae.霍乱弧菌外膜蛋白的组成及免疫化学特性
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Report of the 1966-67 cholera vaccine field trial in rural East Pakistan. 3. The lack of effect of prior vaccination or circulating vibriocidal antibody on the severity of clinical cholera.1966 - 1967年东巴基斯坦农村霍乱疫苗现场试验报告。3. 既往接种疫苗或循环杀弧菌抗体对临床霍乱严重程度的影响缺乏。
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Protective antigens from El Tor vibrios. 1. The preparation and properties of a purified protective antigen from an El Tor vibrio (Ogawa subtype).埃尔托弧菌的保护性抗原。1. 一株埃尔托弧菌（小川亚型）纯化保护性抗原的制备及特性
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Quantitative assay of a protein antigen of vibrio cholerae involved in the vibriocidal action of antibody and complement.霍乱弧菌一种参与抗体和补体杀菌作用的蛋白质抗原的定量测定。
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Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.酶联免疫吸附测定（ELISA）。免疫球蛋白G的定量测定。
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Protection of infant mice against cholera by antibodies to three antigens of Vibrio cholerae.通过针对霍乱弧菌三种抗原的抗体对幼鼠进行霍乱防护。
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Crystalline cholera toxin and toxoid.结晶霍乱毒素和类毒素。
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一种用于研究霍乱弧菌细胞表面抗原的酶联免疫吸附测定法的开发。

Development of an enzyme-linked immunosorbent assay for studying Vibrio cholerae cell surface antigens.

作者信息

Cryz S J, Fürer E, Germanier R

出版信息

J Clin Microbiol. 1982 Jul;16(1):41-5. doi: 10.1128/jcm.16.1.41-45.1982.

DOI:10.1128/jcm.16.1.41-45.1982
PMID:7107860
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC272291/
Abstract

An enzyme-linked immunosorbent assay for the measurement of antibodies directed against cell surface antigens of Vibrio cholerae (CSA ELISA) was developed. NaN3-killed whole cells of V. cholerae, adsorbed to polystyrene tubes, were used as immobilized antigens. The assay was capable of detecting antibodies directed against lipopolysaccharide and non-lipopolysaccharide surface antigens. In addition, the CSA ELISA was capable of detecting non-vibriocidal antibody. An antiserum raised in rabbits by immunization with live V. cholerae 1418 (Ogawa, El Tor) was capable of reacting with various heterologous strains of V. cholerae used as immobilized antigens. Therefore, common antigens shared by V. cholerae strains could be detected by using the CSA ELISA.

摘要

开发了一种用于测量针对霍乱弧菌细胞表面抗原的抗体的酶联免疫吸附测定法（CSA ELISA）。吸附在聚苯乙烯管上的经叠氮化钠灭活的霍乱弧菌全细胞用作固定抗原。该测定法能够检测针对脂多糖和非脂多糖表面抗原的抗体。此外，CSA ELISA能够检测非杀弧菌抗体。通过用活的霍乱弧菌1418（小川型，埃尔托生物型）免疫家兔产生的抗血清能够与用作固定抗原的各种霍乱弧菌异源菌株发生反应。因此，使用CSA ELISA可以检测霍乱弧菌菌株共有的共同抗原。