D'Halluin J C, Milleville M
Biochim Biophys Acta. 1984 May 15;782(1):67-75. doi: 10.1016/0167-4781(84)90107-6.
A method was developed to determine the rate of viral DNA synthesis initiation in adenovirus 2-infected cells. The initiation of DNA synthesis appeared as the rate-limiting step for accumulation of viral DNA. The multiplicity of infection slightly influenced the rate of synthesis of viral DNA, and only during the linear phase of viral DNA production. The initiation of DNA-synthesis was found to occur preferentially on newly synthesized DNA molecules. These kinetics data and the effect of novobiocin suggested that binding of viral DNA with some enzymatic complexes favored the replication of a minor, active class of adenovirus DNA molecules.
已开发出一种方法来测定腺病毒2感染细胞中病毒DNA合成起始的速率。DNA合成的起始似乎是病毒DNA积累的限速步骤。感染复数对病毒DNA的合成速率有轻微影响，且仅在病毒DNA产生的线性阶段如此。发现DNA合成的起始优先发生在新合成的DNA分子上。这些动力学数据和新生霉素的作用表明，病毒DNA与某些酶复合物的结合有利于一类少量活跃的腺病毒DNA分子的复制。
