阿非科林对腺病毒DNA合成的影响。
The effect of aphidicolin on adenovirus DNA synthesis.
作者信息
Longiaru M, Ikeda J E, Jarkovsky Z, Horwitz S B, Horwitz M S
出版信息
Nucleic Acids Res. 1979 Jul 25;6(10):3369-86. doi: 10.1093/nar/6.10.3369.
Abstract
Aphidicolin inhibits adenovirus DNA replication in HeLa cells and in a cell-free, infected, nuclear extract in which viral DNA is elongated. The compound inhibits alpha DNA polymerase, extensively purified from HeLa cells, but has little or no effect on the beta or gamma DNA polymerases similarly purified. Aphidicolin does not affect thymidine uptake by cells nor does synthesis as it also inhibits DNA replication in uninfected cells. The inhibition by aphidicolin is reversible if the drug is removed within 18 hrs after addition to HeLa or Chinese Hamster Ovary cells but the cells are irreversibly affected if the drug remains for 48 hours.
摘要
阿非科林可抑制腺病毒在HeLa细胞以及一种无细胞、受感染的核提取物中的DNA复制，在该核提取物中病毒DNA会发生延长。该化合物可抑制从HeLa细胞中大量纯化得到的α-DNA聚合酶，但对同样纯化得到的β或γ-DNA聚合酶几乎没有影响。阿非科林不影响细胞对胸苷的摄取，也不影响其合成，因为它也会抑制未感染细胞中的DNA复制。如果在向HeLa细胞或中国仓鼠卵巢细胞中添加阿非科林后的18小时内将药物去除，其抑制作用是可逆的，但如果药物作用48小时，细胞就会受到不可逆的影响。
相似文献
1
The effect of aphidicolin on adenovirus DNA synthesis.阿非科林对腺病毒DNA合成的影响。
Nucleic Acids Res. 1979 Jul 25;6(10):3369-86. doi: 10.1093/nar/6.10.3369.
2
Differential effect of aphidicolin on adenovirus DNA synthesis and cellular DNA synthesis.阿非科林对腺病毒DNA合成和细胞DNA合成的差异作用。
Nucleic Acids Res. 1980 Sep 11;8(17):3993-4007. doi: 10.1093/nar/8.17.3993.
3
Involvement of both DNA polymerases alpha and gamma in the replication of adenovirus deoxyribonucleic acid in vitro.DNA聚合酶α和γ参与腺病毒脱氧核糖核酸的体外复制。
Virology. 1980 Jul 15;104(1):56-72. doi: 10.1016/0042-6822(80)90365-7.
4
The effect of aphidicolin on DNA synthesis in isolated HeLa cell nuclei.阿非科林对分离的HeLa细胞核中DNA合成的影响。
Nucleic Acids Res. 1979 Apr;6(4):1583-90. doi: 10.1093/nar/6.4.1583.
5
Involvement of eucaryotic deoxyribonucleic acid polymerases alpha and gamma in the replication of cellular and viral deoxyribonucleic acid.真核生物脱氧核糖核酸聚合酶α和γ参与细胞和病毒脱氧核糖核酸的复制。
Biochemistry. 1979 Oct 2;18(20):4431-43. doi: 10.1021/bi00587a025.
6
Inhibition of DNA synthesis in the adenovirus DNA replication complex by aphidicolin and 2',3'-dideoxythymidine triphosphate.抑癌菌素和2',3'-双脱氧胸苷三磷酸对腺病毒DNA复制复合物中DNA合成的抑制作用。
Biochem Biophys Res Commun. 1980 Jan 15;92(1):8-12. doi: 10.1016/0006-291x(80)91511-9.
7
Replication of adenovirus DNA-protein complex with purified proteins.用纯化蛋白对腺病毒DNA-蛋白质复合物进行复制。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):884-8. doi: 10.1073/pnas.78.2.884.
8
The DNA polymerases associated with the adenovirus type 2 replication complex: effect of 2'-3'-dideoxythymidine-5'-triphosphate on viral DNA synthesis.与2型腺病毒复制复合体相关的DNA聚合酶：2'-3'-双脱氧胸苷-5'-三磷酸对病毒DNA合成的影响
Nucleic Acids Res. 1979 Mar;6(3):1025-39. doi: 10.1093/nar/6.3.1025.
9
Control of cell division by aphidicolin without adverse effects upon resting cells.使用阿非迪霉素控制细胞分裂，且对静止细胞无不良影响。
Arzneimittelforschung. 1985;35(7):1108-16.
10
Characterization of the effect of aphidicolin on adenovirus DNA replication: evidence in support of a protein primer model of initiation.阿非科林对腺病毒DNA复制影响的特征：支持引发蛋白引物模型的证据
Nucleic Acids Res. 1981 Oct 10;9(19):4919-38. doi: 10.1093/nar/9.19.4919.
引用本文的文献
1
Identification of G1-regulated genes in normally cycling human cells.正常循环的人类细胞中G1调控基因的鉴定。
PLoS One. 2008;3(12):e3943. doi: 10.1371/journal.pone.0003943. Epub 2008 Dec 15.
2
Inhibition of adenovirus DNA synthesis in vitro by sera from patients with systemic lupus erythematosus.系统性红斑狼疮患者血清对腺病毒DNA体外合成的抑制作用。
Mol Cell Biol. 1982 Dec;2(12):1492-500. doi: 10.1128/mcb.2.12.1492-1500.1982.
3
DNA, protein, and plasma-membrane incorporation by arrested mammalian cells.停滞的哺乳动物细胞对DNA、蛋白质和质膜的摄取
J Membr Biol. 1994 Oct;142(1):77-92. doi: 10.1007/BF00233385.
4
Initiation of adenovirus DNA replication.腺病毒DNA复制的起始
J Virol. 1980 Sep;35(3):662-71. doi: 10.1128/JVI.35.3.662-671.1980.
5
Chinese hamster ovary cells replicate adenovirus deoxyribonucleic acid.中国仓鼠卵巢细胞复制腺病毒脱氧核糖核酸。
Mol Cell Biol. 1981 Mar;1(3):208-15. doi: 10.1128/mcb.1.3.208-215.1981.
6
Separation of the adenovirus terminal protein precursor from its associated DNA polymerase: role of both proteins in the initiation of adenovirus DNA replication.腺病毒末端蛋白前体与其相关DNA聚合酶的分离：两种蛋白在腺病毒DNA复制起始中的作用
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5225-9. doi: 10.1073/pnas.79.17.5225.
7
Adenovirus DNA replication in vitro: purification of the terminal protein in a functional form.腺病毒DNA的体外复制：功能性末端蛋白的纯化
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6779-83. doi: 10.1073/pnas.78.11.6779.
8
Synthesis of parvovirus H-1 replicative form from viral DNA by DNA polymerase gamma.由DNA聚合酶γ从病毒DNA合成细小病毒H-1复制型。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6206-10. doi: 10.1073/pnas.78.10.6206.
9
Formation of a covalent complex between the 80,000-dalton adenovirus terminal protein and 5'-dCMP in vitro.80,000道尔顿腺病毒末端蛋白与5'-脱氧胞苷酸在体外形成共价复合物。
Proc Natl Acad Sci U S A. 1981 May;78(5):2678-82. doi: 10.1073/pnas.78.5.2678.
10
DNA replication in short-time organ cultures of human epidermis. Inhibition by aphidicolin, and detection of DNA polymerases alpha, beta, and gamma.人表皮短期器官培养中的DNA复制。阿非科林的抑制作用以及DNA聚合酶α、β和γ的检测。
Arch Dermatol Res. 1982;273(1-2):97-101. doi: 10.1007/BF00509032.
本文引用的文献
1
Enzymatic synthesis of deoxyribonucleic acid. II. General properties of the reaction.脱氧核糖核酸的酶促合成。II. 反应的一般性质。
J Biol Chem. 1958 Jul;233(1):171-7.
2
Reconstitution of bacterial DNA-dependent RNA-polymerase from isolated subunits as a tool for the elucidation of the role of the subunits in transcription.从分离的亚基重构细菌DNA依赖性RNA聚合酶作为阐明亚基在转录中作用的工具。
FEBS Lett. 1970 Dec;11(3):165-168. doi: 10.1016/0014-5793(70)80519-1.
3
The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12.腺病毒的多肽。I. 病毒体中多种蛋白质成分的证据以及2型、7A 型和12型的比较
Virology. 1968 Sep;36(1):115-25. doi: 10.1016/0042-6822(68)90121-9.
4
Intermediates in the synthesis of type 2 adenovirus deoxyribonucleic acid.2型腺病毒脱氧核糖核酸合成中的中间体
J Virol. 1971 Nov;8(5):675-83. doi: 10.1128/JVI.8.5.675-683.1971.
5
Intracellular degradation of HeLa and adenovirus type 2 DNA induced by camptothecin.喜树碱诱导的HeLa细胞和2型腺病毒DNA的细胞内降解
Biochem Biophys Res Commun. 1971 Nov 5;45(3):723-7. doi: 10.1016/0006-291x(71)90476-1.
6
Studies on camptothecin. I. Effects of nucleic acid and protein synthesis.喜树碱的研究。I. 核酸与蛋白质合成的影响。
Mol Pharmacol. 1971 Nov;7(6):632-44.
7
The interrelation between DNA synthesis and various DNA polymerase activities in synchronized HeLa cells.同步化的海拉细胞中DNA合成与各种DNA聚合酶活性之间的相互关系。
J Mol Biol. 1974 Jun 15;86(1):11-20. doi: 10.1016/s0022-2836(74)80003-3.
8
Induction of DNA polymerase in mouse L cells.小鼠L细胞中DNA聚合酶的诱导
J Mol Biol. 1973 Feb 15;74(1):1-8. doi: 10.1016/0022-2836(73)90349-5.
9
Variation of deoxyribonucleic acid polymerase activities during rat liver regeneration.大鼠肝脏再生过程中脱氧核糖核酸聚合酶活性的变化
J Biol Chem. 1972 Dec 25;247(24):7948-50.
10
Antiviral effects of aphidicolin, a new antibiotic produced by Cephalosporium aphidicola.头孢蚜霉菌产生的一种新型抗生素——阿非科林的抗病毒作用
Antimicrob Agents Chemother. 1973 Sep;4(3):294-8. doi: 10.1128/AAC.4.3.294.
Zotero 插件