Astier C, Elmorjani K, Meyer I, Joset F, Herdman M
J Bacteriol. 1984 May;158(2):659-64. doi: 10.1128/jb.158.2.659-664.1984.
Among a wide range of potential selective agents examined, sodium p-hydroxymercuribenzoate successfully enriched for mutants of Synechocystis sp. strains PCC 6714 and 6803 defective in photosynthesis. When both photosystems I and II were operating, viability of wild-type cells decreased to between 5 X 10(-5) and 1 X 10(-6) after 5 h of incubation with 500 microM p-hydroxymercuribenzoate (strain 6714), and after 8 h with 200 microM (strain 6803). Between 0.1 and 0.5% of the survivors were stable mutants defective in different steps of photosynthesis. The compound was not mutagenic. It was less toxic to cells grown chemoheterotrophically in the dark or photoheterotrophically in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea. p-Hydroxymercuribenzoate therefore killed only cells which were performing photosynthesis at high rates, thereby specifically selecting for mutants deficient in this process.
在所检测的多种潜在选择剂中，对羟基汞苯甲酸钠成功富集了集胞藻属（Synechocystis sp.）PCC 6714和6803菌株中光合作用缺陷的突变体。当光系统I和II都起作用时，野生型细胞在与500微摩尔对羟基汞苯甲酸钠孵育5小时后（6714菌株），存活率降至5×10⁻⁵至1×10⁻⁶之间，在与200微摩尔孵育8小时后（6803菌株）。0.1%至0.5%的存活者是光合作用不同步骤存在缺陷的稳定突变体。该化合物不具有诱变性。它对在黑暗中进行化学异养生长或在3-(3,4-二氯苯基)-1,1-二甲基脲存在下进行光异养生长的细胞毒性较小。因此，对羟基汞苯甲酸钠仅杀死那些以高速率进行光合作用的细胞，从而特异性地选择出该过程存在缺陷的突变体。
