集胞藻属蓝细菌PCC 6714和PCC 6803菌株的光合突变体：对羟基汞苯甲酸钠作为一种选择剂
Photosynthetic mutants of the cyanobacteria Synechocystis sp. strains PCC 6714 and PCC 6803: sodium p-hydroxymercuribenzoate as a selective agent.
作者信息
Astier C, Elmorjani K, Meyer I, Joset F, Herdman M
出版信息
J Bacteriol. 1984 May;158(2):659-64. doi: 10.1128/jb.158.2.659-664.1984.
Abstract
Among a wide range of potential selective agents examined, sodium p-hydroxymercuribenzoate successfully enriched for mutants of Synechocystis sp. strains PCC 6714 and 6803 defective in photosynthesis. When both photosystems I and II were operating, viability of wild-type cells decreased to between 5 X 10(-5) and 1 X 10(-6) after 5 h of incubation with 500 microM p-hydroxymercuribenzoate (strain 6714), and after 8 h with 200 microM (strain 6803). Between 0.1 and 0.5% of the survivors were stable mutants defective in different steps of photosynthesis. The compound was not mutagenic. It was less toxic to cells grown chemoheterotrophically in the dark or photoheterotrophically in the presence of 3-(3,4-dichlorophenyl)-1,1-dimethylurea. p-Hydroxymercuribenzoate therefore killed only cells which were performing photosynthesis at high rates, thereby specifically selecting for mutants deficient in this process.
摘要
在所检测的多种潜在选择剂中，对羟基汞苯甲酸钠成功富集了集胞藻属（Synechocystis sp.）PCC 6714和6803菌株中光合作用缺陷的突变体。当光系统I和II都起作用时，野生型细胞在与500微摩尔对羟基汞苯甲酸钠孵育5小时后（6714菌株），存活率降至5×10⁻⁵至1×10⁻⁶之间，在与200微摩尔孵育8小时后（6803菌株）。0.1%至0.5%的存活者是光合作用不同步骤存在缺陷的稳定突变体。该化合物不具有诱变性。它对在黑暗中进行化学异养生长或在3-(3,4-二氯苯基)-1,1-二甲基脲存在下进行光异养生长的细胞毒性较小。因此，对羟基汞苯甲酸钠仅杀死那些以高速率进行光合作用的细胞，从而特异性地选择出该过程存在缺陷的突变体。
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本文引用的文献
1
Contributions of Respiratory and Photosynthetic Pathways during Growth of a Facultative Photoautotrophic Cyanobacterium, Aphanocapsa 6714.兼性光合蓝藻 Aphanocapsa 6714 生长过程中呼吸和光合途径的贡献。
Plant Physiol. 1981 Oct;68(4):974-8. doi: 10.1104/pp.68.4.974.
2
Photosynthesis by isolated chloroplasts. VII. Vitamin K and riboflavin phosphate as cofactors of cyclic photophosphorylation.离体叶绿体的光合作用。VII. 维生素K和磷酸核黄素作为循环光合磷酸化的辅助因子。
Biochim Biophys Acta. 1959 Mar;32(1):32-46. doi: 10.1016/0006-3002(59)90550-5.
3
Photosynthesis by isolated chloroplasts. IV. General concept and comparison of three photochemical reactions.离体叶绿体的光合作用。IV. 三种光化学反应的一般概念及比较
Biochim Biophys Acta. 1956 Jun;20(3):449-61. doi: 10.1016/0006-3002(56)90339-0.
4
Metronidazole and the isolation of temperature-sensitive photosynthetic mutants in cyanobacteria.甲硝唑与蓝细菌中温度敏感型光合突变体的分离
J Bioenerg Biomembr. 1980 Aug;12(3-4):277-95. doi: 10.1007/BF00744689.
5
Difference spectra and extinction coefficients of P 700 .P700的差示光谱和消光系数
Biochim Biophys Acta. 1972 Apr 20;267(1):160-71. doi: 10.1016/0005-2728(72)90147-8.
6
A rapid procedure for selective enrichment of photosynthetic electron transport mutants.一种用于光合电子传递突变体选择性富集的快速方法。
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7
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8
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