Dzelzkalns V A, Owens G C, Bogorad L
Nucleic Acids Res. 1984 Dec 11;12(23):8917-25. doi: 10.1093/nar/12.23.8917.
The putative promoter region of the chloroplast encoded ps2B gene (the gene encoding the 32kD herbicide binding B protein of photosystem II (1-4)) has been fused to a chloramphenicol acetyl transferase (CAT) gene that lacks its bacterial promoter and found to accurately initiate transcription from this promoter when introduced into the cyanobacterium, Anacystis nidulans R2 (or into E. coli). The chloroplast promoter-CAT fusion was introduced into the cells on a plasmid that contains plasmid replication origins for E. coli and Anacystis as well as a second antibiotic resistance marker. Cells transformed with corresponding vectors lacking the promoter region do not express CAT.
叶绿体编码的ps2B基因（编码光系统II的32kD除草剂结合B蛋白的基因(1 - 4)）的假定启动子区域已与一个氯霉素乙酰转移酶（CAT）基因融合，该CAT基因缺乏其细菌启动子，并且发现当导入蓝细菌集胞藻6803 R2（或大肠杆菌）时，能从该启动子准确起始转录。叶绿体启动子 - CAT融合基因通过一个质粒导入细胞，该质粒含有大肠杆菌和集胞藻的质粒复制起点以及第二个抗生素抗性标记。用缺乏启动子区域的相应载体转化的细胞不表达CAT。
