Chan M M, Bias W B, Hsu S H, Meyers D A
Proc Natl Acad Sci U S A. 1984 Jun;81(11):3521-5. doi: 10.1073/pnas.81.11.3521.
Vigorous lymphoproliferative responses to synthetic polypeptides poly(L-phenylalanine, L-glutamic acid)-poly(DL-alanine)--poly(L-lysine) [( Phe,G)-A--L], and L-glutamic acid, L-alanine, L-tyrosine (60:30:10) (GAT) were observed in cells from 92 unrelated subjects. Thirty-three percent responded to (Phe,G)-A--L and 77% to GAT. No HLA association was observed with responses to these two antigens. Family studies indicated that two complementary immune response (Ir) genes are required for response to each antigen. Eleven matings were informative for linkage analysis between HLA and these Ir genes. Families in which the complementary genes are in coupling gave maximal lod scores (log of the odds) of 4.50 for (Phe,G)-A--L and 7.57 for GAT for 0 = 0. In a HLA-B/D recombinant family, the Ir- PheGAL genes are mapped towards the HLA-D region. The localization of Ir-GAT genes close to HLA-B was provided by a HLA-A/B recombinant.
在92名无亲缘关系的受试者的细胞中观察到了对合成多肽聚（L-苯丙氨酸，L-谷氨酸）-聚（DL-丙氨酸）-聚（L-赖氨酸）[（Phe，G）-A-L]以及L-谷氨酸、L-丙氨酸、L-酪氨酸（60:30:10）（GAT）的强烈淋巴细胞增殖反应。33%的受试者对（Phe，G）-A-L有反应，77%的受试者对GAT有反应。未观察到HLA与对这两种抗原的反应之间存在关联。家系研究表明，对每种抗原的反应需要两个互补的免疫反应（Ir）基因。有11个交配组合可用于HLA与这些Ir基因之间的连锁分析。互补基因处于顺式排列的家系中，对于（Phe，G）-A-L，θ = 0时最大优势对数（lod）得分为4.50，对于GAT则为7.57。在一个HLA-B/D重组家系中，Ir-PheGAL基因被定位到靠近HLA-D区域。一个HLA-A/B重组体提供了Ir-GAT基因靠近HLA-B的定位信息。
