Hsu S H, Chan M M, Bias W B
Proc Natl Acad Sci U S A. 1981 Jan;78(1):440-4. doi: 10.1073/pnas.78.1.440.
Vigorous lymphocyte proliferative response to synthetic polypeptides was observed in cells from 50 normal volunteers. Results indicated that 64% responded to poly(LHis, LGlu)-poly(DLAla)--poly(LLys) [(H, G)-A--L] and 54% to poly(LTyr, LGlu)-poly(DLAla)--poly(LLys) [(T, G)-A--L]. Subjects could be classified into high-, intermediate-, and non-responder phenotypes according to their stimulation indices. Family studies indicated that high responses to these antigens are inherited as histocompatibility antigen gene (HLA)-linked dominant traits. Two matings suggested gene complementation in response to (T, G)-A--L and (H, G)-A--L. One, with an intra-HLA recombinant offspring, provided evidence localizing the immune response gene(s) controlling lymphocyte proliferation to (T, G)-A--L and (H, G)-A--L, presumably the homologue to Ir-1 of mouse, closer to the HLA-B than to the HLA-D region.
在50名正常志愿者的细胞中观察到对合成多肽有强烈的淋巴细胞增殖反应。结果表明，64%的人对聚（L-组氨酸，L-谷氨酸）-聚（D-丙氨酸）-聚（L-赖氨酸）[（H，G）-A-L]有反应，54%的人对聚（L-酪氨酸，L-谷氨酸）-聚（D-丙氨酸）-聚（L-赖氨酸）[（T，G）-A-L]有反应。根据刺激指数，受试者可分为高反应型、中等反应型和无反应型表型。家族研究表明，对这些抗原的高反应性作为与组织相容性抗原基因（HLA）连锁的显性性状遗传。两个交配组合提示了对（T，G）-A-L和（H，G）-A-L反应中的基因互补。其中一个交配组合产生了一个HLA内部重组后代，提供了证据，将控制淋巴细胞增殖的免疫反应基因定位到（T，G）-A-L和（H，G）-A-L，推测是小鼠Ir-1的同源物，更靠近HLA-B区域而不是HLA-D区域。
