Sobel J D, Obedeanu N
Ann Clin Lab Sci. 1984 May-Jun;14(3):216-24.
Agglutination of yeast, human group A and guinea pig erythrocytes by multiple clinical isolates of E. coli, K. pneumoniae, and P. aeruginosa was investigated and correlated with hydrophobicity measurements of each bacterial strain. Hydrophobicity of the isolates, as measured by hydrophobic interaction chromatography, was similarly correlated with in vitro adherence of the microorganisms to buccal epithelial cells. Agglutination and adherence studies were done in the presence and absence of 0.046 M D (+) mannose. Results showed a wide variability of these parameters among the three general of bacteria. Although E. coli designated mannose sensitive by agglutination showed significantly greater hydrophobicity and attachment to buccal cells, there was no direct correlation between hydrophobic retention and adherence to epithelial cells (p greater than 0.5). As a group, K pneumoniae strains adhered in higher numbers than other gram negative species, but this was unrelated to the hydrophobicity or the designated mannose sensitive/mannose resistant adhesin status of the strain. P. aeruginosa isolates failed to agglutinate yeast and erythrocytes and also adhered poorly to buccal cells. A relationship between bacterial hydrophobicity and in vitro adherence was not found.
研究了大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌的多个临床分离株对酵母、人A组和豚鼠红细胞的凝集作用，并将其与各菌株的疏水性测量结果进行关联。通过疏水相互作用色谱法测量，分离株的疏水性同样与微生物在体外对颊上皮细胞的黏附相关。在存在和不存在0.046 M D(+)甘露糖的情况下进行了凝集和黏附研究。结果表明，这三个细菌属的这些参数存在很大差异。虽然通过凝集确定为甘露糖敏感的大肠杆菌表现出明显更高的疏水性和对颊细胞的附着，但疏水保留与对上皮细胞的黏附之间没有直接相关性(p大于0.5)。作为一个群体，肺炎克雷伯菌菌株比其他革兰氏阴性菌黏附的数量更多，但这与菌株的疏水性或指定的甘露糖敏感/甘露糖抗性黏附素状态无关。铜绿假单胞菌分离株未能凝集酵母和红细胞，对颊细胞的黏附也很差。未发现细菌疏水性与体外黏附之间的关系。
