Thunberg T, Ahlborg U G, Wahlström B
Arch Toxicol. 1984 Mar;55(1):16-9. doi: 10.1007/BF00316580.
The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,7,8- tetrabromodibenzo -p-dioxin ( TBrDD ), 5-chloro-2-(2,4-dichlorophenoxy)phenol (3-Cl- predioxin ) 4,5,6-trichloro-2-(2,4-dichlorophenoxy)phenol (5-Cl- predioxin ), toxaphene, 3-methylcholanthrene (3-MC) and phenobarbital (PB) on the vitamin A storage, UDP-glucuronosyltransferase (UDPGT) and aryl hydrocarbon hydroxylase (AHH) activities in the liver of Sprague-Dawley rats was investigated. Vitamin A was determined as retinol by high pressure liquid chromatography. UDPGT was measured with p-nitrophenol as an aglycone and AHH with 3,4-benzopyrene as a substrate. Both in TCDD- and toxaphene-treated animals a reduced body weight gain was recorded, but no other overt signs of toxicity were seen in this study. Both the concentration and the total amount of hepatic retinol was significantly reduced in TCDD-, 3-MC-, PB- and TBrDD -treated animals. These compounds were also those which gave the most significant enzyme induction as regards the UDPGT and AHH activities. However, the reduction of hepatic retinol caused by these compounds did not correlate with the enzyme activities studied. When compared on a molecular basis, TCDD and TBrDD were in the order of several magnitudes more potent as reducers of hepatic retinol and likewise as enzyme inducers.
研究了2,3,7,8-四氯二苯并 -p-二噁英（TCDD）、2,3,7,8-四溴二苯并 -p-二噁英（TBrDD）、5-氯-2-(2,4-二氯苯氧基)苯酚（3-氯前二噁英）、4,5,6-三氯-2-(2,4-二氯苯氧基)苯酚（5-氯前二噁英）、毒杀芬、3-甲基胆蒽（3-MC）和苯巴比妥（PB）对Sprague-Dawley大鼠肝脏中维生素A储存、尿苷二磷酸葡萄糖醛酸基转移酶（UDPGT）和芳烃羟化酶（AHH）活性的影响。通过高压液相色谱法将维生素A测定为视黄醇。以对硝基苯酚为糖苷配基测定UDPGT，以3,4-苯并芘为底物测定AHH。在TCDD和毒杀芬处理的动物中均记录到体重增加减少，但在本研究中未观察到其他明显的毒性迹象。在TCDD、3-MC、PB和TBrDD处理的动物中，肝脏视黄醇的浓度和总量均显著降低。就UDPGT和AHH活性而言，这些化合物也是诱导酶活性最显著的物质。然而，这些化合物引起的肝脏视黄醇减少与所研究的酶活性无关。在分子基础上进行比较时，TCDD和TBrDD作为肝脏视黄醇的还原剂以及酶诱导剂，其效力要高出几个数量级。
