采用高分辨率气相色谱-质谱联用技术对动物组织中花生四烯酸代谢谱进行表征。

Characterization of arachidonic acid metabolic profiles in animal tissues by high-resolution gas chromatography-mass spectrometry.

作者信息

Chiabrando C, Noseda A, Novella Castagnoli M, Salmona M, Fanelli R

出版信息

Biochim Biophys Acta. 1984 Jul 6;794(2):292-7. doi: 10.1016/0005-2760(84)90158-9.

Abstract

A standardized, highly specific routine method was developed for the quantitative profiling of cyclooxygenase metabolites of arachidonic acid in animal tissues. Whole homogenates were used to assess the potential capacity of tissues to metabolize endogenous arachidonic acid. Samples were analyzed by high-resolution gas chromatography-mass spectrometry in the selected ion monitoring mode. The screening of several rat tissues by this method revealed marked tissue-specificity in both the synthesis capacity and prostaglandin profile. The major products detected were: 6-ketoprostaglandin F1alpha for lung, stomach, muscle and heart; prostaglandin D2 for spleen, brain and liver; prostaglandin F2alpha for kidney and prostaglandin E2 for seminal vesicles. Marked species differences were found when guinea pig tissues were analyzed.

摘要

开发了一种标准化、高特异性的常规方法，用于对动物组织中花生四烯酸的环氧化酶代谢产物进行定量分析。使用全组织匀浆来评估组织代谢内源性花生四烯酸的潜在能力。样品通过高分辨率气相色谱-质谱联用仪在选择离子监测模式下进行分析。用该方法对几种大鼠组织进行筛查，结果显示在合成能力和前列腺素谱方面均存在明显的组织特异性。检测到的主要产物为：肺、胃、肌肉和心脏组织中的6-酮前列腺素F1α；脾脏、脑和肝脏组织中的前列腺素D2；肾脏组织中的前列腺素F2α；精囊组织中的前列腺素E2。分析豚鼠组织时发现了明显的种属差异。