Castelli M G, Butti G, Chiabrando C, Cozzi E, Fanelli R, Gaetani P, Silvani V, Paoletti P
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
J Neurooncol. 1987;5(4):369-75. doi: 10.1007/BF00148394.
We determined arachidonic acid (AA) cyclooxygenase metabolic profiles in specimens of human intracranial tumors (gliomas and meningiomas) and, when available, normal brain tissue. Samples were collected at surgery and immediately frozen in liquid nitrogen. The five stable metabolites of AA (PGE2, PGD2, PGF2 alpha, 6-keto-PGF1 alpha and TXB2) were measured by high-resolution gas chromatography-mass spectrometry after ex vivo metabolism of endogenous AA by tissue homogenates. The absolute amounts of AA metabolites varied widely between samples, though meningiomas and gliomas showed characteristic profiles. Compared to the slow-growing benign meningiomas, the rapidly-growing infiltrating gliomas had higher synthesis of TXA2 (reported as a procancer metabolite) and lower synthesis of PGD2 and PGI2 (reported as anticancer metabolites). A higher overall synthesis capacity, preferentially toward TXA2, was found in glioblastomas than in non-pathological brain tissue.
我们测定了人类颅内肿瘤（胶质瘤和脑膜瘤）标本以及（如有）正常脑组织中花生四烯酸（AA）的环氧化酶代谢谱。样本在手术时采集，并立即在液氮中冷冻。通过组织匀浆对内源性AA进行体外代谢后，采用高分辨率气相色谱 - 质谱法测定AA的五种稳定代谢产物（PGE2、PGD2、PGF2α、6 - 酮 - PGF1α和TXB2）。尽管脑膜瘤和胶质瘤呈现出特征性谱图，但样本之间AA代谢产物的绝对量差异很大。与生长缓慢的良性脑膜瘤相比，生长迅速的浸润性胶质瘤中血栓素A2（TXA2，一种促癌代谢产物）的合成较高，而前列腺素D2（PGD2）和前列环素（PGI2，抗癌代谢产物）的合成较低。与非病理性脑组织相比，胶质母细胞瘤中发现具有更高的总体合成能力，且优先合成TXA2。
