Parente L, Di Rosea M, Flower R J, Ghiara P, Meli R, Persico P, Salmon J A, Wood J N
Eur J Pharmacol. 1984 Mar 23;99(2-3):233-9. doi: 10.1016/0014-2999(84)90246-2.
Glucocorticoid-induced anti-phospholipase proteins were partially purified by using ion-exchange and molecular sieve chromatography. These proteins, as well as dexamethasone itself, inhibited the hind-paw rat oedema induced by carrageenin. This inhibition was reversed by arachidonic acid, Anti-phospholipase proteins as well as hydrocortisone, also reduced the formation of prostaglandin E2 and leukotriene B4 by phagocytosing leucocytes. A specific monoclonal antibody was able to reverse the inhibition of eicosanoid formation. The mechanism of the anti-inflammatory effect of glucocorticoids and anti-phospholipase proteins is discussed in the light of these results.
通过离子交换和分子筛色谱法对糖皮质激素诱导的抗磷脂酶蛋白进行了部分纯化。这些蛋白以及地塞米松本身，均可抑制角叉菜胶诱导的大鼠后爪水肿。花生四烯酸可逆转这种抑制作用。抗磷脂酶蛋白以及氢化可的松，还可减少吞噬白细胞产生前列腺素E2和白三烯B4。一种特异性单克隆抗体能够逆转类花生酸生成的抑制作用。根据这些结果，对糖皮质激素和抗磷脂酶蛋白的抗炎作用机制进行了讨论。
