Russell L M, Cryz S J, Holmes R K
Infect Immun. 1984 Jul;45(1):143-9. doi: 10.1128/iai.45.1.143-149.1984.
During growth under conditions of iron deprivation, Corynebacterium diphtheriae secreted a siderophore into the culture medium. This extracellular siderophore was necessary for rates of iron uptake at pH 8.0 by C. diphtheriae C7 and related strains. We isolated a mutant of C. diphtheriae C7(beta), strain HC6, which did not make the corynebacterial siderophore. Strain HC6 grew very poorly, even under high-iron conditions, and had a severe defect in iron transport. Both growth and iron uptake by strain HC6 were greatly stimulated by the corynebacterial siderophore. We used strain HC6 to develop a bioassay for the corynebacterial siderophore and to look for other potential siderophores for C. diphtheriae. Among the purified phenolate and hydroxamate siderophores tested, only aerobactin was able to stimulate the growth of strain HC6. Partial purification of the corynebacterial siderophore was achieved. The siderophore did not give positive reactions in the Arnow test for phenolates or the Csaky test for hydroxamates and may have a novel chemical structure.
在缺铁条件下生长时，白喉棒状杆菌会向培养基中分泌一种铁载体。这种细胞外铁载体对于白喉棒状杆菌C7及相关菌株在pH 8.0时的铁摄取速率是必需的。我们分离出了白喉棒状杆菌C7（β）的一个突变体，即HC6菌株，它不产生棒状杆菌铁载体。HC6菌株生长非常缓慢，即使在高铁条件下也是如此，并且在铁转运方面存在严重缺陷。棒状杆菌铁载体极大地刺激了HC6菌株的生长和铁摄取。我们利用HC6菌株开发了一种针对棒状杆菌铁载体的生物测定法，并寻找白喉棒状杆菌的其他潜在铁载体。在所测试的纯化酚盐和异羟肟酸铁载体中，只有气杆菌素能够刺激HC6菌株的生长。实现了棒状杆菌铁载体的部分纯化。该铁载体在用于酚盐的阿诺夫试验或用于异羟肟酸的察基试验中均未给出阳性反应，可能具有一种新颖的化学结构。
