Huber C, Batchelor J R, Fuchs D, Hausen A, Lang A, Niederwieser D, Reibnegger G, Swetly P, Troppmair J, Wachter H
J Exp Med. 1984 Jul 1;160(1):310-6. doi: 10.1084/jem.160.1.310.
Neopterin, a compound derived from GTP, represents a precursor molecule of biopterin that is an essential cofactor in neurotransmitter synthesis. We have recently reported that in vivo as well as in vitro immune responses are accompanied by an increased release of neopterin and that this phenomenon can be used for the biochemical monitoring of diseases accompanied by hyperimmune stimulation. This article deals with the cellular origin and the control of this immune response-associated neopterin release in vitro. Using highly purified or monoclonal cellular reagents we demonstrate that macrophages (M phi) stimulated with supernatants from activated T cells release large amounts of neopterin into culture supernatants. Further experiments involving induction of neopterin release from M phi with various human recombinant interferons (IFNs) or neutralization of the effect of T cell supernatants with various monoclonal anti-IFN antibodies revealed immune IFN as the active principle. It thus appears that a metabolic pathway so far exclusively known in context with the generation of an essential cofactor of neurotransmitter-synthesis during immune responses is also activated in M phi under stringent control by immune IFN-like lymphokines.
新蝶呤是一种由鸟苷三磷酸衍生而来的化合物，是生物蝶呤的前体分子，而生物蝶呤是神经递质合成中必不可少的辅助因子。我们最近报道，体内和体外免疫反应均伴随着新蝶呤释放的增加，并且这种现象可用于对伴有免疫亢进刺激的疾病进行生化监测。本文探讨了体外这种与免疫反应相关的新蝶呤释放的细胞来源及其调控。使用高度纯化的或单克隆细胞试剂，我们证明用活化T细胞的上清液刺激的巨噬细胞（M phi）会将大量新蝶呤释放到培养上清液中。进一步的实验，包括用各种人重组干扰素（IFN）诱导M phi释放新蝶呤，或用各种单克隆抗IFN抗体中和T细胞上清液的作用，结果表明免疫干扰素是活性成分。因此，看来迄今为止仅在免疫反应期间神经递质合成的必需辅助因子产生过程中已知的一条代谢途径，在免疫干扰素样淋巴因子的严格控制下，也在M phi中被激活。
