Rosenberg G T, Ross S C, Osborn T G
J Rheumatol. 1984 Jun;11(3):318-20.
Morphologic and physiologic pulmonary changes have been described in the tight-skin (TSK) mouse, but no biochemical studies have been done. We performed qualitative and quantitative analyses of glycosaminoglycans (GAG) on lungs of the TSK mouse. Total GAG were determined by cetylpyridinium chloride precipitation in whole lung specimens from TSK mice and normal (N) mice of different age groups: 1, 6, and 12 months old. There was no significant difference in total GAG, GAG concentration, or dry lung weight between TSK and N mice in any of the 3 age groups. In addition, fractionation of the GAG by electrophoresis revealed similar amounts of individual GAG (predominantly dermatan sulfate and heparin) in TSK and N mice within each age group. Therefore, structural abnormalities in the TSK mouse lung were not demonstrated to be due to qualitative or quantitative changes in GAG.
已在紧皮（TSK）小鼠中描述了肺部的形态学和生理学变化，但尚未进行生化研究。我们对TSK小鼠的肺组织进行了糖胺聚糖（GAG）的定性和定量分析。通过十六烷基吡啶氯化物沉淀法测定了来自不同年龄组（1个月、6个月和12个月大）的TSK小鼠和正常（N）小鼠全肺标本中的总GAG。在这3个年龄组中的任何一组，TSK小鼠和N小鼠之间的总GAG、GAG浓度或肺干重均无显著差异。此外，通过电泳对GAG进行分级分离显示，每个年龄组的TSK小鼠和N小鼠中，各单个GAG（主要是硫酸皮肤素和肝素）的含量相似。因此，未证明TSK小鼠肺部的结构异常是由GAG的定性或定量变化所致。
