Yayoshi M, Araake M, Hayatsu E, Kawakubo Y, Yoshioka M
Microbiol Immunol. 1984;28(3):303-10. doi: 10.1111/j.1348-0421.1984.tb00682.x.
Hemolysis mutants were produced by treating Mycoplasma pneumoniae FH-P24 strain with N-methyl-N-nitro-nitrosoguanidine and were classified into three different groups. The first group of mutants, strains P24-L1, L2, and L11, showed wide and clear hemolytic zones. Their attachment ability to erythrocytes of various animals and to hamster lung cells were the same as those of the parent strain. The second group, strain P24-S1, showed non-hemolysis and non-hemadsorption, but retained the attachment ability to lung cells, although not to erythrocytes. The third group, strain P24-S11, was non-hemolytic, had completely lost the attaching ability, and did not proliferate in vivo. Strains in the first group produced significant microscopic pneumonic lesions in hamsters while strain P24-S1 produced milder lung lesions. Strain P24-S11 did not cause any lung lesions, and organisms were not recovered from the lungs of hamsters. The attachment of M. pneumoniae to respiratory epithelium as a cause of infection and the existence of a relationship between the hemolytic abilities of the organisms and histopathogenicity in the hamster lung tissue were further supported by the present data. It was also shown that the use of hemolysis mutants is useful for the elucidation of pathogenesis in mycoplasma infections.
通过用N-甲基-N-硝基-亚硝基胍处理肺炎支原体FH-P24菌株产生了溶血突变体，并将其分为三个不同的组。第一组突变体，即P24-L1、L2和L11菌株，显示出宽而清晰的溶血区。它们对各种动物红细胞和仓鼠肺细胞的附着能力与亲本菌株相同。第二组，即P24-S1菌株，表现为不溶血和不血细胞吸附，但保留了对肺细胞的附着能力，尽管对红细胞没有附着能力。第三组，即P24-S11菌株，不溶血，完全丧失了附着能力，并且在体内不增殖。第一组菌株在仓鼠中产生了明显的显微镜下肺炎病变，而P24-S1菌株产生的肺部病变较轻。P24-S11菌株没有引起任何肺部病变，并且在仓鼠肺部未检测到该生物体。本数据进一步支持了肺炎支原体附着于呼吸道上皮作为感染原因以及生物体溶血能力与仓鼠肺组织组织致病性之间存在关联。还表明，使用溶血突变体有助于阐明支原体感染的发病机制。
