Yayoshi M
Yale J Biol Med. 1983 Sep-Dec;56(5-6):685-9.
Three different groups of hemolysis mutants were produced by treatment of the M. pneumoniae FH-P24 strain with N-methyl-N-nitro-nitrosoguanidine. The first group of mutants, strains P24-L1, L2, and L11, showed wide and clear hemolytic zones, and attaching ability to erythrocytes and to hamster lung cells were the same as the properties of the parent strain and produced significant microscopic lung lesions. Mutant P24-S1 showed non-hemolysis and non-hemadsorption, yet retained the attaching ability to lung cells and produced milder lung lesions. Mutant P24-S11 showed none of those activities, did not cause any lung lesion, and was never recovered from the lungs of hamsters. A close relationship between the hemolytic ability of M. pneumoniae and the histopathogenicity in the hamster lung is suggested in this study. The attaching ability of organisms seems to be an important factor at the initial stage of infection.
用N-甲基-N-硝基-亚硝基胍处理肺炎支原体FH-P24菌株，产生了三组不同的溶血突变体。第一组突变体，即P24-L1、L2和L11菌株，显示出宽而清晰的溶血区，其对红细胞和仓鼠肺细胞的附着能力与亲本菌株相同，并产生明显的显微镜下肺损伤。突变体P24-S1表现为不溶血和不血细胞吸附，但仍保留对肺细胞的附着能力，并产生较轻的肺损伤。突变体P24-S11不表现出这些活性，不引起任何肺损伤，并且从未在仓鼠肺中检出。本研究提示肺炎支原体的溶血能力与仓鼠肺中的组织致病性密切相关。生物体的附着能力似乎是感染初始阶段的一个重要因素。
