用乙二胺四乙酸预处理大肠杆菌CM891对其对多种标准诱变剂敏感性的影响。

The effect of pretreatment of Escherichia coli CM891 with ethylenediaminetetraacetate on sensitivity to a variety of standard mutagens.

作者信息

Mitchell I D, Gilbert P J

出版信息

Mutat Res. 1984 May;140(1):13-9. doi: 10.1016/0165-7992(84)90065-4.

Abstract

The effect of EDTA pretreatment on the sensitivity of E. coli CM891 to 10 standard mutagens was assayed for cytotoxicity, trp- reversion and mutation to A2Cr . There was no obvious correlation of effect with molecular weight but good correlation with water solubility. EDTA pretreatment did not improve the sensitivity of E. coli to agents which were very soluble in water viz. MMS, CP, ACR, DR and GM. However, with very water-insoluble agents, EDTA pretreatment usually gave a significant, 2-5-fold increase in mutation, viz. with 2NF , BP and CAP (trp locus) but not with 9AA . 2AP , which was moderately soluble in distilled water, only showed a small significant increase at the A2C locus after EDTA pretreatment. Increases in mutation were not always paralleled by increases in cytotoxicity.

摘要

测定了乙二胺四乙酸（EDTA）预处理对大肠杆菌CM891对10种标准诱变剂的敏感性，包括细胞毒性、色氨酸回复突变和向A2Cr的突变。效应与分子量没有明显相关性，但与水溶性有良好相关性。EDTA预处理并未提高大肠杆菌对极易溶于水的试剂（即甲基磺酸甲酯（MMS）、环磷酰胺（CP）、丙烯酰胺（ACR）、丝裂霉素D（DR）和链脲佐菌素（GM））的敏感性。然而，对于极难溶于水的试剂，EDTA预处理通常会使突变显著增加2至5倍，例如对于2-硝基芴（2NF）、苯并[a]芘（BP）和氯霉素（CAP，色氨酸位点），但对9-氨基吖啶（9AA）则不然。2-氨基嘌呤（2AP）在蒸馏水中溶解度适中，EDTA预处理后仅在A2C位点显示出小幅显著增加。突变增加并不总是与细胞毒性增加平行。