Mortelmans K, Riccio E S
SRI International, Biopharmaceutical Development Division, Molecular and Genetic Toxicology Program, 333 Ravenswood Avenue, Menlo Park, CA 94025-3493, USA.
Mutat Res. 2000 Nov 20;455(1-2):61-9. doi: 10.1016/s0027-5107(00)00076-2.
The Escherichia coli WP2 tryptophan reverse mutation assay detects trp(-) to trp(+) reversion at a site blocking a step in the biosynthesis of tryptophan prior to the formation of anthranilic acid. The different WP2 strains all carry the same AT base pair at the critical mutation site within the trpE gene. The assay is currently used by many laboratories in conjunction with the Ames Salmonella assay for screening chemicals for mutagenic activity. In general the WP2 strains are used as a substitute for, or as an addition to Salmonella strain TA102 which also carries an AT base pair at the mutation site. The assay is also recommended together with the Ames assay for data submission to regulatory agencies. National and international guidelines have been established for performing these mutagenicity assays. The E. coli WP2 assay procedures are the same as those described elsewhere in this volume for the Ames Salmonella assay (Mortelmans and Zeiger, 2000) with the exception that limited tryptophan instead of limited histidine is used. This chapter is an addendum to the previous chapter and the reader should refer to the previous chapter for details regarding experimental procedures and assay design.
大肠杆菌WP2色氨酸回复突变试验检测在邻氨基苯甲酸形成之前阻断色氨酸生物合成步骤的位点处从trp(-)到trp(+)的回复突变。不同的WP2菌株在trpE基因内的关键突变位点均携带相同的AT碱基对。目前许多实验室将该试验与艾姆斯沙门氏菌试验结合使用，以筛选具有诱变活性的化学物质。一般来说，WP2菌株用作沙门氏菌TA102菌株的替代品或补充，TA102菌株在突变位点也携带一个AT碱基对。该试验也与艾姆斯试验一起被推荐用于向监管机构提交数据。已经制定了国家和国际准则来进行这些致突变性试验。大肠杆菌WP2试验程序与本卷其他地方所述的艾姆斯沙门氏菌试验程序相同（Mortelmans和Zeiger，2000），不同之处在于使用的是有限的色氨酸而非有限的组氨酸。本章是上一章的附录，读者应参考上一章获取有关实验程序和试验设计的详细信息。
