Klein F, Ricketts R T, Rohrer T R, Jones W I, Clark P M, Flickinger M C
Appl Environ Microbiol. 1984 May;47(5):1023-6. doi: 10.1128/aem.47.5.1023-1026.1984.
A pilot-plant-scale operation was used for studying membrane ultrafiltration and concentration of kiloliter quantities of the lymphokine interleukin-3 with a single set of membranes. Initial use of ammonium sulfate precipitation of interleukin-3 proved erratic in the recovery of biological activity and resulted in corrosion of the processing equipment. Membrane ultrafiltration proved to be effective in enabling control of the degree of concentration and predicting recovery of the biologically active protein.
采用中试规模的操作，用单组膜研究了千升量淋巴因子白细胞介素-3的膜超滤和浓缩过程。最初使用硫酸铵沉淀白细胞介素-3，结果发现生物活性回收率不稳定，并且导致加工设备腐蚀。事实证明，膜超滤能够有效控制浓缩程度并预测生物活性蛋白的回收率。
