Hafen E, Kuroiwa A, Gehring W J
Cell. 1984 Jul;37(3):833-41. doi: 10.1016/0092-8674(84)90418-5.
The locus fushi tarazu appears to be involved in the establishment of the segmentation pattern of the Drosophila embryo. The cuticle of ftz mutant embryos is missing structures in alternating segments such that only half the normal number of segments are present. We have localized ftz+ transcripts in tissue sections of wild-type Drosophila embryos by in situ hybridization. Transcripts from the ftz+ gene were first detected during nuclear cleavage prior to cell formation. During the last two nuclear divisions ftz+ transcription becomes gradually restricted such that at the cellular blastoderm stage the ftz+ transcripts are localized in seven evenly spaced bands of cells. The size of each band is similar to the size of the segment primordia at the blastoderm. By the time segmentation becomes morphologically distinct ftz+ transcripts are no longer detected. These results suggest that the ftz+ gene plays a key role in the determination of the segmentation pattern in the embryo.
成对规则基因座似乎参与了果蝇胚胎节段模式的建立。ftz突变体胚胎的表皮在交替节段中缺少结构，以至于仅存在正常节段数目的一半。我们通过原位杂交在野生型果蝇胚胎的组织切片中定位了ftz+转录本。ftz+基因的转录本在细胞形成之前的核分裂期间首次被检测到。在最后两次核分裂期间，ftz+转录逐渐受到限制，以至于在细胞胚盘阶段，ftz+转录本定位在七个均匀间隔的细胞带中。每个带的大小与胚盘处节段原基的大小相似。到节段在形态上变得明显时，ftz+转录本就不再被检测到了。这些结果表明，ftz+基因在胚胎节段模式的确定中起关键作用。
