Lynd L, Kerby R, Zeikus J G
J Bacteriol. 1982 Jan;149(1):255-63. doi: 10.1128/jb.149.1.255-263.1982.
The Marburg strain of Butyribacterium methylotrophicum did not grow on CO alone but did consume CO during growth on a variety of substrates in the presence of a 100% CO gas phase. We selected a strain (the CO strain) that grew vigorously on CO alone. The ability of the CO strain to grow on CO was stable through multiple transfers in the absence of CO. CO dehydrogenase activity was lower in the CO strain grown on CO (13.3 micromol/min per mg of protein) than in the Marburg strain grown on methanol-acetate (47.2 mumol/min per mg of protein); thus, the levels of this enzyme did not explain the growth on CO. CO was dissimilated to acetate and CO2 with the following stoichiometry: 4 CO leads to 2.17 CO2 + 0.74 acetate. We observed a growth rate of 0.05 h-1, a final optical density at 660 nm of 0.8, and a cell yield of 3.0 g of cells per mol of CO during growth of the CO strain. Growing cultures of the CO strain displayed a Ks for CO of 28 to 56 microM. The apparent thermodynamic efficiency of cell synthesis from CO was 57%. Energetic and biochemical aspects of CO metabolism are described.
甲基营养丁酸杆菌的马尔堡菌株不能仅在一氧化碳上生长，但在100%一氧化碳气相存在下，在多种底物上生长时会消耗一氧化碳。我们筛选出了一种能仅在一氧化碳上旺盛生长的菌株（一氧化碳菌株）。该一氧化碳菌株在无一氧化碳的情况下经过多次传代后，在一氧化碳上生长的能力依然稳定。在一氧化碳上生长的一氧化碳菌株中，一氧化碳脱氢酶活性（每毫克蛋白质13.3微摩尔/分钟）低于在甲醇 - 乙酸盐上生长的马尔堡菌株（每毫克蛋白质47.2微摩尔/分钟）；因此，这种酶的水平并不能解释在一氧化碳上的生长情况。一氧化碳以如下化学计量比被异化生成乙酸盐和二氧化碳：4CO→2.17CO₂ + 0.74乙酸盐。在一氧化碳菌株生长过程中，我们观察到其生长速率为0.05 h⁻¹，660纳米处的最终光密度为0.8，每摩尔一氧化碳的细胞产量为3.0克细胞。生长的一氧化碳菌株培养物对一氧化碳的Ks值为28至5 microM。由一氧化碳合成细胞的表观热力学效率为57%。本文描述了一氧化碳代谢的能量和生化方面。
