Schenken R S, Asch R H, Anderson W H
J Clin Endocrinol Metab. 1984 Sep;59(3):436-40. doi: 10.1210/jcem-59-3-436.
Porcine follicular fluid (pFF) contains a nonsteroidal factor(s) that modifies pituitary secretion of both FSH and LH under certain physiological and experimental conditions. The aim of this study was to further define the activity of pFF on basal and pulsatile gonadotropin secretion after bilateral oophorectomy in monkeys. Sexually mature female rhesus monkeys underwent bilateral oophorectomy during the early follicular phase of the menstrual cycle. For 10 consecutive days, beginning on the day of surgery, monkeys were given twice daily sc injections of 2.5 ml normal saline (group I, control; n = 3), 2.5 ml steroid-free pFF (group II; n = 3), or 5.0 ml steroid-free pFF (group III; n = 3). Blood was drawn daily for 32 days, beginning the day before castration. One day before and on days 6 and 20 after castration, monkeys were placed in a restraining chair, and blood samples were drawn every 15 min for 6 h via an indwelling venous catheter. All samples were radioimmunoassayed for FSH and LH. In group I, a significant increase in serum FSH and LH occurred on days 3 and 6 postcastration, respectively. A significant increase in serum FSH did not occur until day 13 postcastration in groups II and III. Serum LH was significantly increased on days 10 and 9 postcastration in groups II and III, respectively. The LH pulse frequency ranged from 60-105 min and did not change after castration in control or pFF-treated animals. The LH pulse increment was significantly increased on day 6 postcastration in group I, but not until day 20 postcastration in groups II and III. Only minor oscillations in FSH were found until day 20 postcastration, at which time there was no significant difference in FSH pulse frequency or increment among groups. These findings demonstrate that a nonsteroidal factor(s) in pFF suppresses basal FSH and LH concentrations and LH pulse increment in monkeys after surgical castration. Further, because LH, but not FSH, concentrations began to increase during pFF treatments, these data suggest a temporal disparity in the effects of pFF on FSH and LH secretory profiles.
猪卵泡液（pFF）含有一种非甾体因子，在某些生理和实验条件下可改变垂体促卵泡素（FSH）和促黄体素（LH）的分泌。本研究的目的是进一步确定pFF对猴子双侧卵巢切除术后基础和脉冲式促性腺激素分泌的影响。性成熟的雌性恒河猴在月经周期的卵泡早期接受双侧卵巢切除术。从手术当天开始，连续10天，猴子每天接受两次皮下注射，分别为2.5毫升生理盐水（第一组，对照组；n = 3）、2.5毫升无类固醇pFF（第二组；n = 3）或5.0毫升无类固醇pFF（第三组；n = 3）。从去势前一天开始，每天采血32天。在去势前一天以及去势后第6天和第20天，将猴子置于约束椅中，通过留置静脉导管每15分钟采集一次血样，共采集6小时。所有样本均采用放射免疫法检测FSH和LH。在第一组中，去势后第3天和第6天血清FSH和LH分别显著升高。在第二组和第三组中，血清FSH直到去势后第13天才显著升高。在第二组和第三组中，血清LH分别在去势后第10天和第9天显著升高。LH脉冲频率在60 - 105分钟之间，去势后对照组或接受pFF治疗的动物中该频率未发生变化。在第一组中，去势后第6天LH脉冲增幅显著增加，但在第二组和第三组中直到去势后第20天才增加。在去势后第20天之前，仅发现FSH有轻微波动，此时各组之间FSH脉冲频率或增幅无显著差异。这些结果表明，pFF中的一种非甾体因子可抑制猴子手术去势后的基础FSH和LH浓度以及LH脉冲增幅。此外，由于在pFF治疗期间LH浓度开始升高，而FSH浓度未升高，这些数据表明pFF对FSH和LH分泌模式的影响存在时间差异。
