Whateley T L, Steele G, Urwin J, Smail G A
J Clin Hosp Pharm. 1984 Jun;9(2):113-26. doi: 10.1111/j.1365-2710.1984.tb01067.x.
The determination of the particle size of Intralipid fat emulsions by the non-perturbing method of photon correlation spectroscopy has been extended to include particle size determination using the Coulter counter and optical microscopy. Although little increase in particle size in 3-litre bags containing total parenteral nutrition mixtures was observed with the methods involving large dilutions, creaming was observed and optical microscopy showed aggregation and some coalescence to have taken place. The problem of re-dispersion upon dilution of aggregated (but not coalesced) fat emulsions makes direct optical observation essential in assessing the stability and safety of such fat emulsion mixtures. The effect of electrolytes (NaCl and CaCl2) on creaming, particle size and electrophoretic mobility showed both minimum stability and minimum zeta potential at 3 X 10(-3) mol dm-3 CaCl2 and at 2.5 X 10(-1) mol dm-3 NaCl. Thus, these maximum levels for electrolytes in total parenteral mixtures are indicated.
通过光子相关光谱的非扰动方法测定英脱利匹特脂肪乳剂的粒径，已扩展到包括使用库尔特计数器和光学显微镜测定粒径。尽管在含有全胃肠外营养混合物的3升袋中，采用大量稀释的方法观察到粒径几乎没有增加，但观察到了乳析现象，光学显微镜显示发生了聚集和一些聚结。聚集（但未聚结）的脂肪乳剂稀释时重新分散的问题，使得直接光学观察对于评估此类脂肪乳剂混合物的稳定性和安全性至关重要。电解质（氯化钠和氯化钙）对乳析、粒径和电泳迁移率的影响表明，在3×10⁻³摩尔/立方分米氯化钙和2.5×10⁻¹摩尔/立方分米氯化钠时，稳定性和ζ电位均最低。因此，指明了全胃肠外混合物中这些电解质的最大含量。
