Suzuki F, Pollard R B
J Interferon Res. 1984 Spring;4(2):223-33. doi: 10.1089/jir.1984.4.223.
As reported previously, gamma interferon (IFN-gamma) production was selectively decreased in thermally injured mice (TI-Mice) and spleen cell cultures from the mice following stimulation with various IFN inducers. The decrease in IFN production was associated with splenic suppressor macrophages. The present study demonstrated that TI-Mice treated with Ge-132 (TGe-Mice) produced IFN following stimulation with IFN-gamma inducers. The level of IFN activity detected in the sera of TGe-Mice approximated that of controls. Similar results were obtained when spleen cells prepared from TGe-Mice were stimulated with IFN-gamma inducers in vitro. While transfer of spleen cells from TI-Mice resulted in the suppression of IFN production in normal mice (N-Mice) stimulated with IFN-gamma inducers, the transfer of spleen cells derived from TGe-Mice did not induce suppression of IFN production in N-Mice. Mononuclear cells (MNC) prepared from N-Mice produced IFN following concanavalin A (Con A) stimulation when they were co-cultured with macrophage-enriched populations (PAC) obtained from the spleens of TGe-Mice. In contrast, MNC stimulated with Con A did not produce IFN activity when they were co-cultured with PAC of TI-Mice. These results suggest that the generation of suppressor macrophages in TI-Mice may be altered by the administration of Ge-132.
如先前报道，热损伤小鼠（TI小鼠）以及用各种干扰素诱导剂刺激后的小鼠脾细胞培养物中，γ干扰素（IFN-γ）的产生选择性降低。干扰素产生的降低与脾脏抑制性巨噬细胞有关。本研究表明，用Ge-132处理的TI小鼠（TGe小鼠）在用IFN-γ诱导剂刺激后产生干扰素。在TGe小鼠血清中检测到的干扰素活性水平与对照组相近。当用IFN-γ诱导剂在体外刺激从TGe小鼠制备的脾细胞时，也获得了类似结果。虽然将TI小鼠的脾细胞转移会导致用IFN-γ诱导剂刺激的正常小鼠（N小鼠）中干扰素产生受到抑制，但将TGe小鼠来源的脾细胞转移到N小鼠中并不会诱导干扰素产生受到抑制。当从N小鼠制备的单核细胞（MNC）与从TGe小鼠脾脏获得的富含巨噬细胞群体（PAC）共培养时，在伴刀豆球蛋白A（Con A）刺激下会产生干扰素。相反，当MNC与TI小鼠的PAC共培养时，Con A刺激下的MNC不会产生干扰素活性。这些结果表明，给予Ge-132可能会改变TI小鼠中抑制性巨噬细胞的产生。
