Suzuki F, Pollard R B
J Trauma. 1987 Apr;27(4):379-83. doi: 10.1097/00005373-198704000-00006.
A second suppressive state (S-SupS) in interferon (IFN) response was demonstrated when mice with third-degree burns of approximately 30% of the body surface area (TI-mice) were stimulated in vivo by Staphylococcal enterotoxin A, a gamma IFN inducer. The first suppressive state in IFN production, appearing 3 to 7 days after thermal injury, was mediated by the generation of splenic suppressor macrophages. The S-SupS was demonstrated approximately 3 weeks post thermal injury. It persisted for almost 3 weeks and gradually disappeared by 7 weeks. Spleen cells from mice during the S-SupS produced less IFN in vitro than normal mouse splenic mononuclear cells (MNC) when stimulated with concanavalin A (Con A). Splenic MNC of mice during the S-SupS inhibited IFN production when they were co-cultured with normal mouse splenic MNC in the presence of Con A. Since this suppressor cell activity could not be removed from TI-mice splenic MNC by carbonyl iron treatment, or by a technique of adherence to a plastic surface, it is suggested that two different cell populations which are capable of suppressing the IFN response of TI-mice exist at different time periods following burn injury.
当体表约30%面积遭受三度烧伤的小鼠（TI小鼠）在体内受到γ干扰素诱导剂金黄色葡萄球菌肠毒素A刺激时，干扰素（IFN）反应中出现了第二种抑制状态（S-SupS）。热损伤后3至7天出现的IFN产生的第一种抑制状态是由脾脏抑制性巨噬细胞的产生介导的。S-SupS在热损伤后约3周出现。它持续了近3周，并在7周时逐渐消失。在S-SupS期间，用刀豆蛋白A（Con A）刺激时，来自小鼠的脾细胞在体外产生的IFN比正常小鼠脾单核细胞（MNC）少。在Con A存在的情况下，将S-SupS期间小鼠的脾MNC与正常小鼠脾MNC共培养时，前者会抑制IFN的产生。由于这种抑制细胞活性不能通过羰基铁处理或通过粘附于塑料表面的技术从小鼠脾MNC中去除，因此表明在烧伤损伤后的不同时间段存在两种不同的能够抑制小鼠IFN反应的细胞群体。
