Beletskaia L V, Rukosuev V S, Kramerov A A
Arkh Anat Gistol Embriol. 1984 May;86(5):68-72.
By means of concanavalin A (Con A) labelled with fluorescein isotiocyonate in cryostate sections of some human and animal organs (skin, heart, thymus) localization of clycoconjugates containing hexoses and reacting with this lectin has been studied. Glycoconjugates are revealed along the periphery of all cellular elements, in Z-lines and in intercalated disks of the myocardial muscle fibers, in the connective tissue ground substance and also in that of the basal membranes of epithelium, endothelium, muscle fibers (sarcolemma), in cytoplasm of epithelioreticulocytes of the thymus. In fibroblasts of the L-cells culture, glycoconjugates are also localized in cytoplasm. When N-acetylglucosamin is added to the labelled Con A solution, the ability of lectin to react with tissue components is inhibited.
通过用异硫氰酸荧光素标记的伴刀豆球蛋白A（Con A），在一些人和动物器官（皮肤、心脏、胸腺）的冷冻切片中，研究了含有己糖并与这种凝集素反应的糖缀合物的定位。在所有细胞成分的周边、心肌纤维的Z线和闰盘中、结缔组织基质以及上皮、内皮、肌纤维（肌膜）的基底膜中、胸腺上皮网状细胞的细胞质中都发现了糖缀合物。在L细胞培养的成纤维细胞中，糖缀合物也定位在细胞质中。当将N-乙酰葡糖胺添加到标记的Con A溶液中时，凝集素与组织成分反应的能力受到抑制。
