Louis C J, Wyllie R G
Experientia. 1981 May 15;37(5):508-9. doi: 10.1007/BF01986166.
A method is described for using a fluorescein isothiocyanate concanavalin A conjugate to stain human cell membranes in formalin fixed paraffin embedded tissue. 57 neoplastic and normal tissue sites were examined. In 54 malignant tumours, bright green fluorescence was confined to the cell membranes while in 23 benign tumours and normal tissue sites, the membranes were unstained or showed a diminished level of fluorescence. The distinction between malignant and hyperplastic or normal cells was clear cut and definite.
描述了一种使用异硫氰酸荧光素伴刀豆球蛋白A缀合物对福尔马林固定石蜡包埋组织中的人细胞膜进行染色的方法。检查了57个肿瘤和正常组织部位。在54个恶性肿瘤中，亮绿色荧光局限于细胞膜，而在23个良性肿瘤和正常组织部位，细胞膜未染色或荧光水平降低。恶性细胞与增生性或正常细胞之间的区别清晰明确。
