Kouttab N M, Mehta S, Morgan J, Tannir N, Sahasrabuddhe C, Maizel A L
Clin Chem. 1984 Sep;30(9):1539-45.
The development of a competent immunoregulatory response in the face of an antigenic challenge is modulated by soluble proteins of relatively low molecular mass. Lymphokines and monokines, secreted by cells of T lineage and cells of the monocyte/microphage series, respectively, function in a bimodal amplification network that results in the proliferation and differentiation of the immunoregulatory cells. Interleukin 1 is typically assayed by its effect on thymocytes or by its ability to promote the T cell-dependent release of interleukin 2. Interleukin 2 is routinely measured by its ability to support the long-term growth of cultured T cells, whereas B cell growth factor is measured by its ability to support the long-term growth of cultured B lymphocytes. The availability of homogeneous purified factors and the subsequent availability of monoclonal antibodies against these reagents should allow for the development of rapid quantitative assays for these analytes in diverse biological fluids. In addition, large quantities of purified reagents will promote studies to determine therapeutic efficacy in several immunodeficiency syndromes.
面对抗原刺激时，有效的免疫调节反应的发展受到相对低分子量的可溶性蛋白质的调节。分别由T细胞系细胞和单核细胞/巨噬细胞系细胞分泌的淋巴因子和单核因子，在一个双峰放大网络中发挥作用，该网络导致免疫调节细胞的增殖和分化。白细胞介素1通常通过其对胸腺细胞的作用或通过其促进白细胞介素2的T细胞依赖性释放的能力来测定。白细胞介素2通常通过其支持培养的T细胞长期生长的能力来测量，而B细胞生长因子则通过其支持培养的B淋巴细胞长期生长的能力来测量。均一纯化因子的可得性以及随后针对这些试剂的单克隆抗体的可得性，应有助于开发针对多种生物体液中这些分析物的快速定量测定方法。此外，大量纯化试剂将促进在几种免疫缺陷综合征中确定治疗效果的研究。
