Baldaia L, Porcheron P, Coimbra J, Cassier P
Gen Comp Endocrinol. 1984 Sep;55(3):437-43. doi: 10.1016/0016-6480(84)90015-7.
Ecdysteroids were studied during the molt cycle of the shrimp Palaemon serratus using radioimmunoassay and HPLC. Continuous presence of ecdysteroids was observed in whole extracts, with two hormonal peaks: a minor peak during stage B and a major one during stage D2. The ecdysteroid titers were generally higher in females than in males. The ecdysteroid peak at stage B was more pronounced in the integument (cuticle and epidermis) than in other tissues and, at this time, 20-hydroxyecdysone was the predominant form. The observed 20-hydroxyecdysone peaks in stages B and D2 are correlatable to the previously reported rise in integumental carbonic anhydrase activity.
使用放射免疫分析和高效液相色谱法，对锯齿长臂虾蜕皮周期中的蜕皮类固醇进行了研究。在整个提取物中观察到蜕皮类固醇持续存在，有两个激素峰值：B阶段出现一个较小的峰值，D2阶段出现一个较大的峰值。雌性的蜕皮类固醇滴度通常高于雄性。B阶段的蜕皮类固醇峰值在体壁（角质层和表皮）中比在其他组织中更明显，此时，20-羟基蜕皮酮是主要形式。在B阶段和D2阶段观察到的20-羟基蜕皮酮峰值与先前报道的体壁碳酸酐酶活性升高相关。
