Chambers C M, Dotson E M, Oliver J H
Institute of Arthropodology and Parasitology, Georgia Southern University, Statesboro 30460-8056, USA.
J Med Entomol. 1996 Jan;33(1):11-4. doi: 10.1093/jmedent/33.1.11.
Ecdysteroid titers in methanolic extracts of protonymphal and deutonymphal stages of the chicken mite, Dermanyssus gallinae (De Geer), were measured using radioimmunoassay. Highest titers of ecdysteroids were present 24 h after feeding. Further studies using high-performance liquid chromatography radioimmunoassay showed that ecdysteroid immunopositive material had the same retention time as 20-hydroxyecdysone and ecdysone. Ecdysteroid conjugates appear to be present in polar and apolar fractions.
