Johnston M G, Walker M A
In Vitro. 1984 Jul;20(7):566-72. doi: 10.1007/BF02639772.
Endothelial and smooth-muscle cells from bovine mesenteric lymphatic vessels have been collected and cultured in vitro. The endothelial cells grew as a monolayer exhibiting a "cobblestone" appearance with individual cells tending to be more flattened at confluence than their blood vascular counterparts. Approximately 30% of these cells expressed Factor VIII antigen compared with bovine mesenteric artery or human umbilical-vein endothelium in which the majority of cells were positive. The lymphatic smooth-muscle cells exhibited focal areas of multilayering and were Factor VIII negative. The availability of lymphatic endothelial and smooth-muscle cells in culture will provide a new tool for the investigation of the biological properties of the lymphatic vessels and their role in homeostasis.
已从牛肠系膜淋巴管中收集内皮细胞和平滑肌细胞，并进行体外培养。内皮细胞呈单层生长，呈现“鹅卵石”外观，与血管内皮细胞相比，单个细胞在汇合时往往更扁平。与牛肠系膜动脉或人脐静脉内皮细胞（其中大多数细胞呈阳性）相比，这些细胞中约30%表达因子VIII抗原。淋巴平滑肌细胞表现出局部多层区域，且因子VIII呈阴性。培养的淋巴管内皮细胞和平滑肌细胞将为研究淋巴管的生物学特性及其在体内平衡中的作用提供新工具。
