[不同龄期旋毛虫幼虫乳酸脱氢酶同工酶的研究]
[Lactate dehydrogenase isoenzyme study of Trichinella larvae of different ages].
作者信息
Novoselska L, Zhelev Zh, Ivanov Ia
出版信息
Parazitologiia. 1978 Mar-Apr;12(2):131-4.
LDH isoenzymes of Trichinella larvae isolated from experimentally infected guinea pigs were investigated between days 30 and 400 inclusive after infection. It was found that on the 30 th day after infection the larvae have two isoenzymes while from the 40th to the 400 th day--three isoenzymes. The first of the latter is of fast responce and is located in the anod area near the indicator while the two others are of slow response and are located between the isoenzymes LDH-3 and LDH-5 of the muscular tissue of guinea pigs. This indicates that energetic metabolism of Trichinella larvae proceeds in muscular tissues by aerobic and anaerobic way. Since electrophoretic specificity of LDH-isoenzymes of Trichinella larvae differ from that of LDH-isoenzymes of muscular tissue the former are apparently characteristic of Trichinella larvae.
对从实验感染豚鼠中分离出的旋毛虫幼虫的乳酸脱氢酶同工酶进行了研究，研究时间为感染后第30天至第400天（含第30天和第400天）。结果发现，感染后第30天幼虫有两种同工酶，而从第40天至第400天有三种同工酶。后三种同工酶中的第一种反应快，位于指示剂附近的阳极区，另外两种反应慢，位于豚鼠肌肉组织的乳酸脱氢酶-3和乳酸脱氢酶-5同工酶之间。这表明旋毛虫幼虫的能量代谢通过有氧和无氧方式在肌肉组织中进行。由于旋毛虫幼虫乳酸脱氢酶同工酶的电泳特异性与肌肉组织乳酸脱氢酶同工酶不同，前者显然是旋毛虫幼虫的特征。
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