奇异变形杆菌的recA基因对枯草芽孢杆菌recE基因的功能替代

Functional substitution of the recE gene of Bacillus subtilis by the recA gene of Proteus mirabilis.

作者信息

Eitner G, Manteuffel R, Hofemeister J

出版信息

Mol Gen Genet. 1984;195(3):516-22. doi: 10.1007/BF00341456.

Abstract

Rec mutants of Bacillus subtilis have been tested for complementation by the recA gene of Proteus mirabilis (recApm) which was introduced into B. subtilis via the plasmid pHP334. In the recE4 mutant of B. subtilis the plasmid pHP334 restored significantly the defects in RecE functions tested: UV-sensitivity, homologous recombination (transduction and transformation) and prophage induction. Although serological methods to detect the presence of RecApm protein in B. subtilis have been unsuccessful, our results strongly indicate that the recE function of B. subtilis is analogous to the recA function of P. mirabilis.

摘要

已通过质粒pHP334将奇异变形杆菌的recA基因（recApm）导入枯草芽孢杆菌，对枯草芽孢杆菌的Rec突变体进行互补测试。在枯草芽孢杆菌的recE4突变体中，质粒pHP334显著恢复了所测试的RecE功能缺陷：紫外线敏感性、同源重组（转导和转化）以及原噬菌体诱导。尽管检测枯草芽孢杆菌中RecApm蛋白存在的血清学方法未成功，但我们的结果强烈表明，枯草芽孢杆菌的recE功能类似于奇异变形杆菌的recA功能。