Eitner-Mönke G, Manteuffel R
J Basic Microbiol. 1986;26(2):67-74. doi: 10.1002/jobm.3620260202.
The recA gene of Proteus mirabilis (recApm) has been cloned into the PstI site of the Bacillus promoter-probe plasmid pPL603. When present on this plasmid, the recApm1) gene is expressed in B. subtilis under the control of its own transcriptional and translational signals. It is concluded that the high AT-content of the DNA sequence upstream of the -35 region is of decisive importance for the usage of the recApm promoter by the B. subtilis RNA polymerase. The results are discussed in relation to the expression barriers found to exist for genes from gram-negative bacteria in the gram-positive B. subtilis.
奇异变形杆菌的recA基因（recApm）已被克隆到芽孢杆菌启动子探针质粒pPL603的PstI位点。当该基因存在于该质粒上时，recApm基因在其自身转录和翻译信号的控制下在枯草芽孢杆菌中表达。得出的结论是，-35区域上游DNA序列的高AT含量对于枯草芽孢杆菌RNA聚合酶使用recApm启动子至关重要。结合在革兰氏阳性的枯草芽孢杆菌中发现的革兰氏阴性细菌基因存在的表达障碍对结果进行了讨论。
