Stĕrba F
Vet Med (Praha). 1984 Jun;29(6):353-71.
Sixty-four guinea-pigs were infected with the germs of Brucella suis. biotype 2 via the vaginal, peroral, intranasal, conjunctival and subcutaneous routes. In the subcutaneous infection the length of the trial was 151 days and in the other methods of infection 56 days. As found, guinea-pigs are highly sensitive and brucellosis lesions were detected in all cases. Brucellosis was chronical and spread locally in the guinea-pigs. In the haematogenic spreading of the disease, lesions were constantly recorded in liver, spleen and lungs. In the cases of subcutaneous infection lesions were also observed in regional lymph nodes at the places of infection. The lesions had amorphous structure and regular borders. The lesions were characterized by pronounced exudation and the exudate was subject to caseous necrosis, followed by colliquation. Secondary central colliquation was regularly observed in older granulomas, so that colliquation is considered as a characteristic morphological trait of brucellosis process in guinea-pigs. Histiocytary granuloma constituted the microscopic basis of the lesions. In older granulomas the central necroses were wide and contained a large amount of nuclear detritus, owing to the karyorhexis of cells, mainly neutrophiles. In the necroses of caseous type lipoid droplets were found and calcification was observed on the 151st day. Big cells of Langhans type occurred sometimes in the histiocytary layer of granulomas; they were present most frequently in the lesions affecting lymph nodes. Interstitial pneumonia was a characteristic symptom in liver and big cells of Sternberg type were found in the granulomas. Follicular tumor was constantly observed in spleens. Morphologically detectable lesions first developed in liver after about 7 days from conjunctival infection and subcutaneous infection, after 21 days from vaginal and intranasal infection, and after 28 days from peroral infection. In the case of subcutaneous infection, granulomas were found in the regional lymph nodes on the 14th day. The most expressive lesions in organs, mainly liver, were found from the 49th to 56th day from infection.
64只豚鼠通过阴道、经口、鼻内、结膜下和皮下途径感染猪布鲁氏菌生物2型。皮下感染的试验时长为151天，其他感染方法的试验时长为56天。结果发现，豚鼠高度敏感，所有病例均检测到布鲁氏菌病病变。布鲁氏菌病呈慢性，在豚鼠体内局部扩散。在疾病的血源性传播中，肝脏、脾脏和肺部不断出现病变。皮下感染的病例中，感染部位的局部淋巴结也观察到病变。病变具有无定形结构和规则边界。病变的特征是明显的渗出，渗出物发生干酪样坏死，随后液化。在较老的肉芽肿中经常观察到继发性中央液化，因此液化被认为是豚鼠布鲁氏菌病过程的一个特征性形态学特征。组织细胞性肉芽肿构成病变的微观基础。在较老的肉芽肿中，中央坏死区较宽，由于细胞（主要是嗜中性粒细胞）的核碎裂，含有大量核碎屑。在干酪样坏死中发现类脂滴，并在第151天观察到钙化。朗汉斯型大细胞有时出现在肉芽肿的组织细胞层中；它们最常出现在影响淋巴结的病变中。间质性肺炎是肝脏的一个特征性症状，在肉芽肿中发现斯特恩伯格型大细胞。脾脏中经常观察到滤泡性肿瘤。结膜感染和皮下感染后约7天，经阴道和鼻内感染后21天，经口感染后28天，肝脏中首次出现形态学上可检测到的病变。皮下感染的情况下，第14天在局部淋巴结中发现肉芽肿。从感染后第49天到第56天，在器官中，主要是肝脏中发现最明显的病变。
