Gabridge M G
Yale J Biol Med. 1983 Sep-Dec;56(5-6):657-63.
Mycoplasma pneumoniae is a human pathogen of the respiratory tract. It attaches to the ciliated respiratory epithelium by means of its attachment tip and the sialoglycoprotein receptor site on host cells. To study the mechanical and biochemical features of the attachment process, we developed a new in vitro biological model of respiratory tissue. The ciliated monolayer system involved a collagenase treatment of rodent tracheal explants, followed by incubation in Waymouth's MAB 87/3 medium. Epithelial migration led to the development of patches of cell monolayers both interior and exterior to the tracheal lumen. After seven days of incubation, monolayer patches contained 20 percent ciliated cells. Ciliary motion was active for several days after the explant was removed. When exposed to M. pneumoniae for two hours, the ciliated cells became covered with pathogen. The mycoplasmas were in close association with the host cell membranes, and could lie horizontally along the membrane when not physically held in a vertical orientation by cilia. Cytonecrosis developed within 48 to 72 hours.
肺炎支原体是一种人类呼吸道病原体。它通过其附着尖端和宿主细胞上的唾液糖蛋白受体位点附着于呼吸道纤毛上皮。为了研究附着过程的力学和生化特征，我们开发了一种新的呼吸道组织体外生物学模型。纤毛单层系统包括用胶原酶处理啮齿动物气管外植体，然后在韦茅斯MAB 87/3培养基中孵育。上皮迁移导致气管腔内、外均形成细胞单层斑块。孵育7天后，单层斑块中含有20%的纤毛细胞。外植体取出后，纤毛运动活跃了几天。当暴露于肺炎支原体两小时后，纤毛细胞被病原体覆盖。支原体与宿主细胞膜紧密相连，当未被纤毛以垂直方向物理固定时，可沿细胞膜水平排列。48至72小时内出现细胞坏死。
