Dallegri F, Patrone F, Frumento G, Banchi L, Sacchetti C
Acta Haematol. 1984;71(6):371-5. doi: 10.1159/000206621.
Normal human neutrophils, incubated with 0.2 mg/ml opsonized zymosan particles, were found to lyse human (H), ox (O) and chicken (C) red blood cell (RBC) targets as determined by the 51Cr release assay. The susceptibility to the lysis of the different target cells was HRBC less than ORBC less than CRBC. An intact neutrophil metabolic burst was essential for the cytotoxic event, since neutrophils from a patient with chronic granulomatous disease failed to kill all three target cells. HRBC and ORBC destruction was prevented by catalase and unaffected by azide, suggesting the requirement of hydrogen peroxide alone in the lethal hit. CRBC destruction was abolished by catalase and azide, suggesting the involvement of the myeloperoxidase-hydrogen peroxide system. Thus, different neutrophil cytolytic systems may become operative and may vary in their efficiency depending on the type of target cells.
通过51Cr释放试验测定发现，正常人类中性粒细胞与0.2mg/ml调理酵母聚糖颗粒一起孵育后，能够裂解人类（H）、牛（O）和鸡（C）的红细胞（RBC）靶细胞。不同靶细胞对裂解的敏感性为人类红细胞＜牛红细胞＜鸡红细胞。完整的中性粒细胞代谢爆发对于细胞毒性事件至关重要，因为慢性肉芽肿病患者的中性粒细胞无法杀死所有三种靶细胞。过氧化氢酶可阻止人类红细胞和牛红细胞的破坏，而叠氮化物对此无影响，这表明致死性打击仅需要过氧化氢。过氧化氢酶和叠氮化物可消除鸡红细胞的破坏，这表明髓过氧化物酶-过氧化氢系统参与其中。因此，不同的中性粒细胞溶细胞系统可能会起作用，并且其效率可能会因靶细胞类型而异。
