Vicentini L M, Villereal M L
Biochem Biophys Res Commun. 1984 Sep 17;123(2):663-70. doi: 10.1016/0006-291x(84)90280-8.
The mitogens serum, vasopressin and bradykinin stimulate a significant rise in the inositol phosphate content of cultured human fibroblasts within 10 seconds, while serum- and bradykinin-stimulated arachidonic acid release does not occur until after 30 seconds. The release of inositol phosphates is not secondary to a rise in Ca activity since the Ca ionophore ionomycin does not stimulate release of inositol phosphates. Moreover, we show that phospholipase C in human fibroblasts is activated by these mitogens at resting Ca levels since TMB-8, which blocks the mitogen-induced rise in Ca activity, does not affect the serum-stimulated accumulation of inositol phosphates.
促细胞分裂剂血清、血管加压素和缓激肽可在10秒内刺激培养的人成纤维细胞中的肌醇磷酸含量显著升高，而血清和缓激肽刺激的花生四烯酸释放直到30秒后才会发生。肌醇磷酸的释放并非继发于Ca活性的升高，因为钙离子载体离子霉素不会刺激肌醇磷酸的释放。此外，我们发现，在静息Ca水平下，人成纤维细胞中的磷脂酶C可被这些促细胞分裂剂激活，因为可阻断促细胞分裂剂诱导的Ca活性升高的TMB-8并不影响血清刺激的肌醇磷酸积累。
