佛波醇12-肉豆蔻酸酯13-乙酸酯对鸡胚细胞中毒蕈碱受体介导的钙动员的抑制作用。
Inhibition of muscarinic receptor-mediated Ca mobilization by phorbol 12-myristate 13-acetate in chick embryo cells.
作者信息
Schmidt Heinrich, Oettling Günter, Drews Ulrich
机构信息
Anatomisches Institut der Universität Tübingen, Österbergstraße 3, D-7400, Tübingen, Germany.
出版信息
Rouxs Arch Dev Biol. 1988 Jan;197(1):37-39. doi: 10.1007/BF00376039.
A muscarinic cholinergic receptor is present on undifferentiated cells of the chick embryo. Stimulation of the muscarinic receptor with muscarinic agonists triggers intracellular Ca mobilization. Here, we investigate the effect of phorbol 12-myristate 13-acetate (PMA) on the muscarinic receptor-mediated Ca mobilization, which is monitored in cell suspensions of chick embryos of stage 24 by chlorotetracycline fluorescence. PMA inhibits the Ca mobilization in a time-dependent and concentration-dependent manner without changing the ED of acetylcholine. The concentration of PMA that gives halfmaximal inhibition is 3.1×10 M PMA.
毒蕈碱型胆碱能受体存在于鸡胚的未分化细胞上。用毒蕈碱激动剂刺激毒蕈碱受体可触发细胞内钙动员。在此，我们研究佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）对毒蕈碱受体介导的钙动员的影响，该影响通过用氯四环素荧光法在第24阶段鸡胚的细胞悬液中进行监测。PMA以时间和浓度依赖性方式抑制钙动员，而不改变乙酰胆碱的半数有效剂量。产生半数最大抑制作用的PMA浓度为3.1×10⁻⁶M PMA。
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