Ozeki T, Osada K, Seishima M, Mori S, Nozawa Y
Department of Dermatology, Gifu University School of Medicine, Japan.
Arch Dermatol Res. 1994;286(3-4):133-6. doi: 10.1007/BF00374207.
The numbers of bradykinin receptors (BK-R) in cultured dermal fibroblasts from patients with progressive systemic sclerosis (PSS) and from healthy controls were measured using a receptor binding assay. The numbers of BK-R were significantly fewer in PSS fibroblasts than in control fibroblasts (P < 0.02). However, no differences in affinity were observed in BK-R between PSS and control fibroblasts. The BK-R mRNA levels were determined in PSS and control fibroblasts by Northern blot hybridization using BK-R cDNA, but no significant differences were found. These findings suggest that the decrease in BK-R in PSS fibroblasts might occur during a posttranslational step.
采用受体结合试验测定进行性系统性硬化症（PSS）患者和健康对照者培养的皮肤成纤维细胞中缓激肽受体（BK-R）的数量。PSS成纤维细胞中BK-R的数量显著少于对照成纤维细胞（P < 0.02）。然而，PSS和对照成纤维细胞的BK-R亲和力未观察到差异。使用BK-R cDNA通过Northern印迹杂交测定PSS和对照成纤维细胞中的BK-R mRNA水平，但未发现显著差异。这些发现表明，PSS成纤维细胞中BK-R的减少可能发生在翻译后步骤。
