Abbott L B, Lott J A
Clin Chem. 1984 Nov;30(11):1861-3.
Reactivation of serum creatine kinase isoenzyme BB (CK-BB) with 2-mercaptoethanol and EDTA increased the electrophoretic detection rate of CK-BB from 34% to 78% in 58 hospitalized patients with various malignancies. Patients with solid tumors showed the largest and patients with hematologic malignancies the smallest percentage increase in CK-BB after reactivation. For serum from 50 hospitalized patients without cancer, reactivation resulted in detectable CK-BB in two patients; the CK-BB band was never seen in 15 healthy adults. For reasons unknown, five of eight patients with senile dementia of the Alzheimer's type showed CK-BB in serum after reactivation, as did two of five patients suspected of having this disorder. Serum CK-BB may be a useful tumor marker if reactivation with a thiol and EDTA is used immediately after collection.
用2-巯基乙醇和乙二胺四乙酸（EDTA）重新激活血清肌酸激酶同工酶BB（CK-BB）后，58例患有各种恶性肿瘤的住院患者中CK-BB的电泳检测率从34%提高到了78%。实体瘤患者重新激活后CK-BB增加的百分比最大，血液系统恶性肿瘤患者增加的百分比最小。对于50例无癌症的住院患者的血清，重新激活后两名患者检测到了CK-BB；15名健康成年人的血清中从未见过CK-BB条带。原因不明的是，8例阿尔茨海默病型老年痴呆患者中有5例重新激活后血清中出现了CK-BB，5例疑似患有这种疾病的患者中有2例也是如此。如果在采集后立即用硫醇和EDTA进行重新激活，血清CK-BB可能是一种有用的肿瘤标志物。
