Sleigh M J, Both G W, Brownlee G G
Nucleic Acids Res. 1979 Apr;6(4):1309-21. doi: 10.1093/nar/6.4.1309.
Previous estimates of the size of the RNA genome segments of influenza virus have been unreliable because of a lack of suitable RNA species as size markers. We have attempted to overcome this problem by utilising the ability of AMV reverse transcriptase to synthesise full length DNA copies of RNA molecules in the presence of a suitable primer. By comparing such DNA copies of the RNA segments of the influenza virus genome with sequenced restriction fragments from the E. coli plasmid pBR322, we have made more reliable estimates of the sizes of the eight genome segments from influenza virus A/NT/60/68.
由于缺乏合适的RNA种类作为大小标记，以往对流感病毒RNA基因组片段大小的估计并不可靠。我们试图通过利用禽成髓细胞瘤病毒（AMV）逆转录酶在合适引物存在下合成RNA分子全长DNA拷贝的能力来克服这一问题。通过将流感病毒基因组RNA片段的此类DNA拷贝与来自大肠杆菌质粒pBR322的测序限制性片段进行比较，我们对甲型流感病毒A/NT/60/68的八个基因组片段的大小做出了更可靠的估计。
