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相似文献

1
Evidence for allosterism in in vitro DNA synthesis on RNA templates.RNA模板上体外DNA合成中变构作用的证据。
Proc Natl Acad Sci U S A. 1974 Mar;71(3):858-62. doi: 10.1073/pnas.71.3.858.
2
Viral and cellular DNA polymerase: comparison of activities with synthetic and natural RNA templates.病毒和细胞DNA聚合酶：与合成及天然RNA模板的活性比较
Science. 1972 May 19;176(4036):798-800. doi: 10.1126/science.176.4036.798.
3
RNA-directed DNA-polymerase activity in Greene hamster melanoma.格林仓鼠黑色素瘤中的RNA指导的DNA聚合酶活性
Yale J Biol Med. 1973 Dec;46(5):485-91.
4
Synthesis of DNA complementary to cellular slime mold messenger RNA by reverse transcriptase.用逆转录酶合成与细胞黏菌信使核糖核酸互补的脱氧核糖核酸
Biochemistry. 1974 Sep 10;13(19):3917-22. doi: 10.1021/bi00716a016.
5
DNA polymerases from RNA tumor viruses and human cells: inhibition by polyuridylic acid.来自RNA肿瘤病毒和人类细胞的DNA聚合酶：多聚尿苷酸对其的抑制作用
Science. 1972 Sep 22;177(4054):1111-4. doi: 10.1126/science.177.4054.1111.
6
Primer requirement and template specificity of a DNA polymerase of chick embryo.鸡胚DNA聚合酶的引物需求和模板特异性
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1229-33. doi: 10.1073/pnas.70.4.1229.
7
Comparison of biochemical characteristics of reverse transcriptase from human acute leukemic cells and several RNA tumor viruses.人急性白血病细胞与几种RNA肿瘤病毒逆转录酶的生化特性比较。
Basic Life Sci. 1974;3:345-54. doi: 10.1007/978-1-4613-4529-9_27.
8
Accessibility of DNA in chromatin to DNA polymerase and RNA polymerase.染色质中的DNA对DNA聚合酶和RNA聚合酶的可及性。
Proc Natl Acad Sci U S A. 1973 May;70(5):1326-30. doi: 10.1073/pnas.70.5.1326.
9
Reverse transciptase from Mason-Pfizer monkey tumor virus, avian myeloblastosis virus, and Rauscher leukemia virus and its response to rifamycin derivatives.来自梅森- Pfizer猴肿瘤病毒、禽成髓细胞瘤病毒和劳氏肉瘤病毒的逆转录酶及其对利福霉素衍生物的反应。
J Natl Cancer Inst. 1972 Apr;48(4):1185-9.
10
Transcription of in vitro polyadenylated ribonucleic acid with reverse transcriptase.用逆转录酶对体外聚腺苷酸化核糖核酸进行转录。
Biochemistry. 1974 May 7;13(10):2235-40. doi: 10.1021/bi00707a033.

引用本文的文献

1
Synthesis of DNA complementary to the mRNAs for milk proteins by E. coli DNA polymerase I.大肠杆菌DNA聚合酶I合成与乳蛋白mRNA互补的DNA
Nucleic Acids Res. 1976 Mar;3(3):615-30. doi: 10.1093/nar/3.3.615.
2
DNA polymerases from Chlamydomonas reinhardii. Further characterization, action of inhibitors and associated nuclease activities.莱茵衣藻的DNA聚合酶。进一步的特性、抑制剂的作用及相关核酸酶活性
Biochem J. 1978 Apr 1;171(1):241-9. doi: 10.1042/bj1710241.
3
Apparent allosterism by avian myeloblastosis virus reverse transcriptase and E. coli DNA polymerase I.禽成髓细胞瘤病毒逆转录酶和大肠杆菌DNA聚合酶I的明显变构效应
Nucleic Acids Res. 1979 Mar;6(3):1189-201. doi: 10.1093/nar/6.3.1189.
4
E. coli tRNAs as inhibitors of viral reverse transcription in vitro.体外大肠杆菌转运RNA作为病毒逆转录的抑制剂
Nucleic Acids Res. 1975 Dec;2(12):2315-28. doi: 10.1093/nar/2.12.2315.
5
Observations on template-specific conditions for DNA synthesis by avian myeloblastosis virus DNA polymerase.禽成髓细胞瘤病毒DNA聚合酶进行DNA合成的模板特异性条件观察
Nucleic Acids Res. 1976 Jun;3(6):1473-86. doi: 10.1093/nar/3.6.1473.
6
Mechanism of interaction of avian myeloblastosis virus reverse transcriptase with avian myeloblastosis virus RNA.禽成髓细胞瘤病毒逆转录酶与禽成髓细胞瘤病毒RNA的相互作用机制
J Virol. 1976 Apr;18(1):26-33. doi: 10.1128/JVI.18.1.26-33.1976.
7
Deoxyribonucleic acid polymerases of Euglena gracilis. Primer-template utilization of and enzyme activities associated with the two deoxyribonucleic acid polymerases of high molecular weight.纤细裸藻的脱氧核糖核酸聚合酶。两种高分子量脱氧核糖核酸聚合酶的引物-模板利用及相关酶活性
Biochem J. 1975 Nov;151(2):239-47. doi: 10.1042/bj1510239.

本文引用的文献

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The Molecular Weight and Aggregation of DNA.DNA的分子量与聚集
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2
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.脱氧核糖核酸的酶促合成。IX. T2噬菌体感染大肠杆菌后形成的聚合酶：一种新酶。
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T4 bacteriophage gene 32: a structural protein in the replication and recombination of DNA.T4噬菌体基因32：DNA复制与重组中的一种结构蛋白。
Nature. 1970 Sep 26;227(5265):1313-8. doi: 10.1038/2271313a0.
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Enzymatic synthesis of deoxyribonucleic acid. XXX. Binding of triphosphates to deoxyribonucleic acid polymerase.脱氧核糖核酸的酶促合成。XXX. 三磷酸酯与脱氧核糖核酸聚合酶的结合
J Biol Chem. 1969 Jun 10;244(11):3038-44.
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Electron microscopy of DNA polymerase bound to DNA.与DNA结合的DNA聚合酶的电子显微镜观察。
J Mol Biol. 1971 Jan 28;55(2):209-14. doi: 10.1016/0022-2836(71)90192-6.
6
Enzymatic synthesis of deoxyribonucleic acid. XXXV. A 3'-hydroxylribonucleotide binding site of Escherichia coli deoxyribonucleic acid polymerase.
J Biol Chem. 1970 Oct 25;245(20):5326-34.
7
Enzymatic synthesis of deoxyribonucleic acid. XXXI. Binding of deoxyribonucleic acid to deoxyribonucleic acid polymerase.脱氧核糖核酸的酶促合成。第三十一部分。脱氧核糖核酸与脱氧核糖核酸聚合酶的结合。
J Biol Chem. 1969 Jun 10;244(11):3045-52.
8
DNA polymerase: evidence for multiple molecular species.DNA聚合酶：多种分子种类的证据。
Proc Natl Acad Sci U S A. 1968 Mar;59(3):951-8. doi: 10.1073/pnas.59.3.951.
9
The mechanism of Escherichia coli deoxyribonucleic acid polymerase I. Magnetic resonance and kinetic studies of the role of metals.
J Biol Chem. 1972 Nov 10;247(21):6784-94.
10
Purification and characterization of the deoxyribonucleic acid polymerase associated with Rous sarcoma virus.与劳氏肉瘤病毒相关的脱氧核糖核酸聚合酶的纯化与特性分析
Biochemistry. 1972 Jun 6;11(12):2334-42. doi: 10.1021/bi00762a020.

RNA模板上体外DNA合成中变构作用的证据。

Evidence for allosterism in in vitro DNA synthesis on RNA templates.

作者信息

Cavalieri L F, Modak M J, Marcus S L

出版信息

Proc Natl Acad Sci U S A. 1974 Mar;71(3):858-62. doi: 10.1073/pnas.71.3.858.

DOI:10.1073/pnas.71.3.858
PMID:4132534
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388114/
Abstract

Hemoglobin mRNA and (rA)(n).(dT)(10) have been used as primer-templates in a kinetic study of DNA synthesis with Escherichia coli DNA polymerase I (DNA nucleotidyl transferase, EC 2.7.7.7) and Mason-Pfizer monkey virus reverse transcriptase (RNA-directed DNA polymerase). The rate versus enzyme concentration curve is sigmoidal and is consistent with a cooperative phenomenon. The results could be interpreted in terms of the formation of an active complex containing enzyme dimers (or oligomers) on the primer-template. We have also observed sigmoidal kinetics in rate versus deoxynucleotide triphosphate concentration. These results are consistent with an allosteric mechanism in which the triphosphates act as both modifiers and DNA precursors. In the critical range, a 6- to 8-fold increase in both enzyme and triphosphate concentrations can lead to a 1500-fold increase in the rate of synthesis on an RNA template. Thus, small changes in enzyme and precursor concentrations could play a regulatory role in vivo.

摘要

血红蛋白信使核糖核酸（mRNA）和（rA）（n）·（dT）（10）已被用作引物模板，用于对大肠杆菌DNA聚合酶I（DNA核苷酸转移酶，EC 2.7.7.7）和梅森- Pfizer猴病毒逆转录酶（RNA指导的DNA聚合酶）进行DNA合成的动力学研究。速率与酶浓度曲线呈S形，这与协同现象一致。结果可以用在引物模板上形成包含酶二聚体（或寡聚体）的活性复合物来解释。我们还观察到速率与脱氧核苷三磷酸浓度的S形动力学。这些结果与一种变构机制一致，其中三磷酸既作为调节剂又作为DNA前体。在临界范围内，酶和三磷酸浓度均增加6至8倍可导致RNA模板上合成速率增加1500倍。因此，酶和前体浓度的微小变化可能在体内发挥调节作用。