单链DNA“A”和“B”型的螺旋几何结构源自二聚体亚基的最低能量构象。
Helix geometry of single stranded DNA 'A' and 'B' forms from minimum energy conformations of dimeric subunits.
作者信息
Hingerty B, Broyde S
出版信息
Nucleic Acids Res. 1978 Jan;5(1):127-37. doi: 10.1093/nar/5.1.127.
Abstract
Low energy conformations with dihedral angles similar to those occurring in fibers of the 'A' and 'B' forms of DNAs have been calculated for the deoxydinucleoside phosphates dApdA, dCpdC, dTpdT, dGpdG and dGpdC (1-3). These conformers have been used as building blocks for generating larger single stranded polymers, whose helical parameters we have calculated. We find that single stranded 'A' and 'B' form helices tend to be narrower and more tightly wound than the duplexes obtained in fibers (4,5). This is consistent with experimental observations on single stranded fibers of poly (rC) (6). We also find that the different sequences have different helix geometries. In addition, it is observed that large variations in helix geometry for a given sequence are achievable at little energetic cost.
摘要
已针对脱氧二核苷磷酸dApdA、dCpdC、dTpdT、dGpdG和dGpdC（1 - 3）计算出具有与DNA“A”和“B”形式纤维中出现的二面角相似的低能构象。这些构象异构体已被用作构建更大单链聚合物的结构单元，我们已计算出其螺旋参数。我们发现，单链“A”和“B”形式的螺旋往往比纤维中获得的双链体更窄且缠绕更紧密。这与聚（rC）单链纤维的实验观察结果一致（6）。我们还发现不同的序列具有不同的螺旋几何形状。此外，观察到对于给定序列，在几乎不消耗能量的情况下可实现螺旋几何形状的巨大变化。
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