低离子强度下溴化乙锭与合成双链多核苷酸的相互作用。
The interaction of ethidium with synthetic double-stranded polynucleotides at low ionic strength.
作者信息
Baguley B C, Falkenhaug E M
出版信息
Nucleic Acids Res. 1978 Jan;5(1):161-71. doi: 10.1093/nar/5.1.161.
Abstract
The interaction of ethidium with synthetic DNA and RNA double-stranded polymers at 0.01 M ionic strength, pH 7.0, has been studied by fluorimetry at low drug to nucleotide ratios. Binding constants have been calculated assuming an excluded-neighbouring site model for the interaction of ethidium with double-stranded polymers. The values obtained are poly d(AT).poly d(AT), 9.5 X 10(6) M-1; poly dA.poly dT, 6.5 X 10(5) M-1; poly d(GC).poly d(GC), 9.9 X 10(6) M-1; poly dG,poly dC, 4.5 X 1-(6) M-1; poly d(AC); poly d(GT), 9.8 X 10(6) M-1; poly d(AG).poly d(CT), 1.3 X 10(6) M-1; poly rA.poly rU, 4.1 X 10(7) M-1. The displacement of ethidium from poly d(AT).poly d(AT) by 9-aminoacridine and an acridine-containing antitumor agent (NSC 156303; 4'-(9-acridinylamino)methanesulphon-m-anisidide) has also been examined.
摘要
在离子强度0.01M、pH值7.0的条件下，通过荧光分析法研究了在低药物与核苷酸比例时溴化乙锭与合成的DNA和RNA双链聚合物的相互作用。假定溴化乙锭与双链聚合物相互作用的相邻位点排除模型，计算了结合常数。得到的值分别为：聚d(AT)·聚d(AT)，9.5×10⁶M⁻¹；聚dA·聚dT，6.5×10⁵M⁻¹；聚d(GC)·聚d(GC)，9.9×10⁶M⁻¹；聚dG·聚dC，4.5×10⁶M⁻¹；聚d(AC)·聚d(GT)，9.8×10⁶M⁻¹；聚d(AG)·聚d(CT)，1.3×10⁶M⁻¹；聚rA·聚rU，4.1×10⁷M⁻¹。还研究了9-氨基吖啶和一种含吖啶的抗肿瘤剂（NSC 156303；4'-（9-吖啶基氨基）甲磺酰基间甲氧基苯胺）对溴化乙锭从聚d(AT)·聚d(AT)上的取代作用。
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