Blank M L, Robinson M, Fitzgerald V, Snyder F
J Chromatogr. 1984 Aug 31;298(3):473-82. doi: 10.1016/s0021-9673(01)92744-x.
A novel method is described for the quantitative analysis of subclasses (alk-1-enylacyl, alkylacyl, and diacyl types) and molecular species within each subclass of glycerophosphatides. Diradylglycerols from phospholipase C hydrolysis of the phospholipids are converted to benzoate derivatives, the benzoates are separated into their respective subclasses by thin-layer chromatography, and quantitated by measuring absorbance at 230 nm. Molecular species within individual subclasses are separated using a combination of argentation thin-layer chromatography and reversed-phase high-performance liquid chromatography with direct, on-line quantitation at 230 nm. We applied the method to the analysis of ethanolamine phosphatides from beef brain and were able to quantitate the three diradylglycerol subclasses (alk-1-enylacyl, alkylacyl, and diacyl types) as well as ca. 29 molecular species within each of these subclasses. This new quantitative approach for the analysis of specific molecular species of glycerolipids should be applicable to studies involving a variety of biologically important lipids, such as phosphatidylcholine, phosphatidylinositol, platelet activating factor, plasmalogens, and neutral type glycerolipids including diacylglycerols.
本文描述了一种用于定量分析甘油磷脂各亚类（alk-1-烯基酰基、烷基酰基和二酰基类型）及其内部分子种类的新方法。磷脂经磷脂酶C水解产生的二脂酰甘油被转化为苯甲酸酯衍生物，这些苯甲酸酯通过薄层色谱法分离成各自的亚类，并通过测量230nm处的吸光度进行定量。单个亚类中的分子种类通过银化薄层色谱和反相高效液相色谱相结合的方法进行分离，并在230nm处进行直接在线定量。我们将该方法应用于牛脑乙醇胺磷脂的分析，能够定量三种二脂酰甘油亚类（alk-1-烯基酰基、烷基酰基和二酰基类型）以及这些亚类中各约29种分子种类。这种用于分析甘油脂质特定分子种类的新定量方法应适用于涉及多种具有生物学重要性的脂质的研究，如磷脂酰胆碱、磷脂酰肌醇、血小板活化因子、缩醛磷脂以及包括二酰基甘油在内的中性甘油脂质。
