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鞘磷脂合成导致磷脂酰胆碱的利用和二酰甘油的产生。

Utilization of phosphatidylcholine and production of diradylglycerol as a consequence of sphingomyelin synthesis.

作者信息

Sillence D J, Allan D

机构信息

Department of Physiology, University College London, Rockefeller Building, University Street, London WC1E 6JJ, U.K.

出版信息

Biochem J. 1998 Apr 1;331 ( Pt 1)(Pt 1):251-6. doi: 10.1042/bj3310251.

DOI:10.1042/bj3310251
PMID:9512487
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1219346/
Abstract
  1. After the degradation of cell-surface sphingomyelin (SM) by exogenous sphingomyelinase (SMase), the resynthesis of SM by baby-hamster kidney (BHK) and human leukaemia-60 (HL-60) cells was examined in relation to utilization of substrate phosphatidylcholine (PtdCho) and generation of the expected product, diradylglycerol (DRG). Using [3H]choline-labelled BHK cells incubated in non-radioactive medium, SMase caused a release of phosphocholine, which was derived approximately equally from SM and PtdCho, consistent with the anticipated resynthesis of SM at the expense of PtdCho. However, with choline-labelled cells incubated in radioactive medium or [14C]acetate-labelled cells treated with SMase, no loss of radioactivity from PtdCho or accumulation of labelled DRG was observed, suggesting that any DRG produced as a consequence of SM synthesis must have been rapidly converted back into PtdCho. In contrast, SMase treatment of HL-60 cells caused more than a doubling of DRG levels at the expense of PtdCho, and this appears to be the first demonstration of a rise in DRG related to the synthesis of SM. The DRG produced consisted of about 80% 1,2-diacylglycerol and 18% 1-O-alkyl-2-acylglycerol species, a similar composition to that of the DRG backbone of total cell PtdCho. 2. The requirement for cell-surface PtdCho in the biosynthesis of SM by BHK cells was also investigated. Treatment of [3H]choline-labelled BHK cells with Bacillus cereus PtdCho-specific phospholipase C (PLC) rapidly degraded about 6% of the total PtdCho, which was assumed to represent the cell-surface pool. This did not appear to be the pool of PtdCho required for SM synthesis, since (a) the released phosphocholine was additional to that derived from PtdCho in cells treated with SMase and (b) treatment with PLC did not affect SM synthesis, either de novo or in response to degradation of cell-surface SM by SMase. These findings suggest either that there is no SM synthase in the plasma membrane or, if it is present, then it does not utilize cell-surface PtdCho as a substrate.
摘要
  1. 在用外源性鞘磷脂酶（SMase）降解细胞表面鞘磷脂（SM）后，研究了幼仓鼠肾（BHK）细胞和人白血病60（HL-60）细胞中SM的再合成与底物磷脂酰胆碱（PtdCho）的利用以及预期产物二酰基甘油（DRG）生成之间的关系。使用在非放射性培养基中培养的[3H]胆碱标记的BHK细胞，SMase导致磷酸胆碱释放，其大约等量地来源于SM和PtdCho，这与预期的以PtdCho为代价再合成SM一致。然而，对于在放射性培养基中培养的胆碱标记细胞或用SMase处理的[14C]乙酸盐标记细胞，未观察到PtdCho的放射性损失或标记DRG的积累，这表明由于SM合成产生的任何DRG必定已迅速转化回PtdCho。相反，用SMase处理HL-60细胞导致以PtdCho为代价的DRG水平增加了一倍多，这似乎是首次证明与SM合成相关的DRG升高。产生的DRG约由80%的1,2-二酰基甘油和18%的1-O-烷基-2-酰基甘油组成，其组成与总细胞PtdCho的DRG主链相似。2. 还研究了BHK细胞在SM生物合成中对细胞表面PtdCho的需求。用蜡样芽孢杆菌PtdCho特异性磷脂酶C（PLC）处理[3H]胆碱标记的BHK细胞，迅速降解了约6%的总PtdCho，假定其代表细胞表面池。这似乎不是SM合成所需的PtdCho池，因为（a）释放的磷酸胆碱是在用SMase处理的细胞中来源于PtdCho的磷酸胆碱之外的，并且（b）用PLC处理不影响从头合成的SM或对SMase降解细胞表面SM的反应中的SM合成。这些发现表明，要么质膜中不存在SM合酶，要么如果存在，那么它不利用细胞表面PtdCho作为底物。

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本文引用的文献

1
Is plasma membrane lipid composition defined in the exocytic or the endocytic pathway?质膜脂质组成是在胞吐途径还是内吞途径中确定的？
Trends Cell Biol. 1994 Oct;4(10):350-3. doi: 10.1016/0962-8924(94)90076-0.
2
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
3
The subcellular sites of sphingomyelin synthesis in BHK cells.BHK细胞中鞘磷脂合成的亚细胞位点。
Biochim Biophys Acta. 1997 Oct 30;1359(1):1-12. doi: 10.1016/s0167-4889(97)00088-8.
4
Essential role for diacylglycerol in protein transport from the yeast Golgi complex.二酰基甘油在酵母高尔基体复合体蛋白质转运中的重要作用。
Nature. 1997 May 1;387(6628):101-5. doi: 10.1038/387101a0.
5
Sphingomyelin synthase is absent from endosomes.内体中不存在鞘磷脂合酶。
J Cell Sci. 1997 Mar;110 ( Pt 6):781-8. doi: 10.1242/jcs.110.6.781.
6
Phorbol ester-sensitive phospholipase D is mainly localized in the endoplasmic reticulum of BHK cells.佛波酯敏感的磷脂酶D主要定位于BHK细胞的内质网中。
Biochem J. 1996 Dec 15;320 ( Pt 3)(Pt 3):885-90. doi: 10.1042/bj3200885.
7
Mapping the lipid distribution in the membranes of BHK cells (mini-review).绘制BHK细胞细胞膜中的脂质分布（综述）。
Mol Membr Biol. 1996 Apr-Jun;13(2):81-4. doi: 10.3109/09687689609160580.
8
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Biochem Biophys Res Commun. 1996 Aug 14;225(2):392-9. doi: 10.1006/bbrc.1996.1185.
9
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