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参与温度和蜕皮激素诱导转录的序列位于黑腹果蝇热休克基因的不同区域。

Sequences involved in temperature and ecdysterone-induced transcription are located in separate regions of a Drosophila melanogaster heat shock gene.

作者信息

Hoffman E, Corces V

出版信息

Mol Cell Biol. 1986 Feb;6(2):663-73. doi: 10.1128/mcb.6.2.663-673.1986.

DOI:10.1128/mcb.6.2.663-673.1986
PMID:3097502
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367558/
Abstract

The transcriptional regulation of the Drosophila melanogaster hsp27 (also called hsp28) gene was studied by introducing altered genes into the germ line by P element-mediated transformation. DNA sequences upstream of the gene were defined with respect to their effect on steroid hormone-induced and heat-induced transcription. These two types of control were found to be separable; the sequences responsible for 80% of heat-induced expression were located more than 1.1 kilobases upstream of the RNA initiation site, while the sequences responsible for the majority of ecdysterone induction were positioned downstream of the site at -227 base pairs. We have determined the DNA sequence of the intergenic region separating hsp23 and hsp27 and have located putative heat shock and ecdysterone consensus sequences. Our results indicate that the heat shock promoter of the hsp27 gene is organized quite differently from that of hsp70.

摘要

通过P因子介导的转化将改变的基因导入种系，对黑腹果蝇hsp27（也称为hsp28）基因的转录调控进行了研究。根据基因上游DNA序列对类固醇激素诱导转录和热诱导转录的影响来对其进行界定。发现这两种调控方式是可分离的；负责80%热诱导表达的序列位于RNA起始位点上游超过1.1千碱基处，而负责大部分蜕皮甾酮诱导的序列位于该位点下游-227碱基对处。我们确定了分隔hsp23和hsp27的基因间区域的DNA序列，并定位了假定的热休克和蜕皮甾酮共有序列。我们的结果表明，hsp27基因的热休克启动子与hsp70的热休克启动子组织方式有很大不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b90/367558/ba5ee23435d9/molcellb00086-0332-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b90/367558/fb34bf2a7632/molcellb00086-0330-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b90/367558/76008ddb433c/molcellb00086-0331-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b90/367558/78002dd2d6bf/molcellb00086-0331-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b90/367558/ba5ee23435d9/molcellb00086-0332-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b90/367558/fb34bf2a7632/molcellb00086-0330-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b90/367558/76008ddb433c/molcellb00086-0331-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b90/367558/78002dd2d6bf/molcellb00086-0331-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b90/367558/ba5ee23435d9/molcellb00086-0332-a.jpg

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1
Sequences involved in temperature and ecdysterone-induced transcription are located in separate regions of a Drosophila melanogaster heat shock gene.参与温度和蜕皮激素诱导转录的序列位于黑腹果蝇热休克基因的不同区域。
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本文引用的文献

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Developmental regulation of Drosophila imaginal disc proteins: synthesis of a heat shock protein under non-heat-shock conditions.果蝇成虫盘蛋白的发育调控：非热休克条件下热休克蛋白的合成。
Dev Biol. 1983 Feb;95(2):325-30. doi: 10.1016/0012-1606(83)90033-7.
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An expandable gene that encodes a Drosophila glue protein is not expressed in variants lacking remote upstream sequences.一个编码果蝇胶水蛋白的可扩展基因在缺乏远端上游序列的变体中不表达。
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热休克和蜕皮激素激活果蝇 hsp27 启动子需要独立的远程调控序列。
EMBO J. 1986 Jul;5(7):1653-8. doi: 10.1002/j.1460-2075.1986.tb04408.x.
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Decreased survival of mosquito cells after stable transfection with a Drosophila ecdysteroid response element: possible involvement of a 40 kDa DNA binding protein.
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EMBO J. 1997 May 1;16(9):2452-62. doi: 10.1093/emboj/16.9.2452.
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Molecular characterization of an 18 kb segment of DNA puff C4 of Bradysia hygida (Diptera, sciaridae).嗜菌大眼蕈蚊（双翅目，眼蕈蚊科）DNA膨泡C4的18 kb片段的分子特征分析
Chromosoma. 1995 Jul;103(10):715-24. doi: 10.1007/BF00344233.
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A germline transformation analysis reveals flexibility in the organization of heat shock consensus elements.种系转化分析揭示了热休克共有元件组织中的灵活性。
Nucleic Acids Res. 1987 Apr 10;15(7):2971-88. doi: 10.1093/nar/15.7.2971.
8
The ovarian, ecdysterone, and heat-shock-responsive promoters of the Drosophila melanogaster hsp27 gene react very differently to perturbations of DNA sequence.果蝇hsp27基因的卵巢、蜕皮激素和热休克反应性启动子对DNA序列的扰动反应差异很大。
Mol Cell Biol. 1987 Mar;7(3):973-81. doi: 10.1128/mcb.7.3.973-981.1987.
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Organization of the Drosophila melanogaster hsp70 heat shock regulation unit.黑腹果蝇hsp70热休克调节单元的组织
Mol Cell Biol. 1987 Mar;7(3):1055-62. doi: 10.1128/mcb.7.3.1055-1062.1987.
10
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6
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7
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High-resolution mapping of DNase I-hypersensitive sites of Drosophila heat shock genes in Drosophila melanogaster and Saccharomyces cerevisiae.果蝇热休克基因在黑腹果蝇和酿酒酵母中DNase I超敏位点的高分辨率图谱绘制。
Mol Cell Biol. 1984 Sep;4(9):1853-63. doi: 10.1128/mcb.4.9.1853-1863.1984.
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Upstream elements necessary for optimal function of the hsp 70 promoter in transformed flies.转化果蝇中hsp 70启动子最佳功能所需的上游元件。
Cell. 1984 Sep;38(2):391-8. doi: 10.1016/0092-8674(84)90494-x.